Eliminating hydrogen consuming bacteria is a critical step in anaerobic fermentation for biohyclrogen production. Treatment of anaerobic granular sludge with chloroform was reported as effective in transforming a methane-producing system into a hydrogen-producing system by eliminating methane production. This study, using 16S rRNA gene sequences, further assessed changes in microbial community composition as a result of chloroform treatment and during continuous cultivation of chloroform-treated granules in a continuous upflow reactor employing immobilized cells. Profiles of terminal restriction fragment length polymorphisms (T-RFLP) of 16S rRNA genes sequences cloned from samples before and after chloroform treatment showed that metbanogenic hydrogen consumer and Methanosaeta harundinacea sp. were eliminated. Methanosaeta concilii, however, was not eliminated from the hydrogen-producing system, which might explain, in part, the granulation phenomena in the anaerobic hydrogen fermentation system. The results also shoived that Clostridium buryricum dominated the hydrogen-production system.
- 16S rDNA microbial analysis
- Biological hydrogen production
- Chloroform treatment
- Clone library