Changes in acetylcholinesterase activity and muscarinic receptor bindings in μ-opioid receptor knockout mice

Lu Tai Tien, Lir Wan Fan, Chiharu Sogawa, Tangeng Ma, Horace H Loh, Ing Kang Ho

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Anatomical evidence indicates that cholinergic and opioidergic systems are co-localized and acting on the same neurons. However, the regulatory mechanisms between cholinergic and opioidergic system have not been well characterized. In the present study, we investigated whether there are compensatory changes of acetylcholinesterase activity and cholinergic receptors in mice lacking μ-opioid receptor gene. The acetylcholinesterase activity was determined by histochemistry assay. The cholinergic receptor binding was carried out by quantitative autoradiography using [3H]-quinuclidinyl benzilate (nonselective muscarinic receptors), N-[3H]-methylscopolamine (nonselective muscarinic receptors), [3H]-pirenzepine (M1 subtype muscarinic receptors) and [3H]-AF-DX384 (M2 subtype muscarinic receptors) in brain slices of wild-type and μ-opioid receptor knockout mice. The acetylcholinesterase activity of μ-opioid receptor knockout mice was higher than that of the wild-type in the striatal caudate putamen and nucleus accumbens, but not in the cortex and hippocampus areas. In addition, the bindings in N-[3H]-methylscopolamine and [3H]-AF-DX384 of μ-opioid receptor knockout mice were significantly lower when compared with that of the wild-type controls in the striatal caudate putamen and nucleus accumbens. However, there were no significant differences in bindings of [ 3H]-quinuclidinyl benzilate and [3H]-pirenzepine between μ-opioid receptor knockout and wild-type mice in the cortex, striatum and hippocampus. These data indicate that there are up-regulation of acetylcholinesterase activity and compensatory down-regulation of M2 muscarinic receptors in the striatal caudate putamen and nucleus accumbens of μ-opioid receptor knockout mice.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalMolecular Brain Research
Volume126
Issue number1
DOIs
StatePublished - Jul 5 2004

Keywords

  • Acetylcholine receptors: muscarinic
  • Autoradiography
  • Histochemistry
  • M1 and M2 muscarinic receptors
  • Mu-opioid receptor knockout mice
  • Neurotransmitters, modulators, transporters, and receptors

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