Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks

J. Lawrence, E. Karpuzoglu, A. Vance, M. Vandenplas, C. Saba, M. Turek, R. M. Gogal

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.

Original languageEnglish (US)
Pages (from-to)545-548
Number of pages4
JournalResearch in veterinary science
Volume94
Issue number3
DOIs
StatePublished - Jun 1 2013

Keywords

  • Chemiluminescence
  • Feline
  • Immunocytochemistry
  • Tumor
  • Western Blot

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