Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks

J. Lawrence; E. Karpuzoglu; A. Vance; M. Vandenplas; C. Saba; M. Turek; R. M. Gogal

doi:10.1016/j.rvsc.2013.01.007

Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks

J. Lawrence, E. Karpuzoglu, A. Vance, M. Vandenplas, C. Saba, M. Turek, R. M. Gogal

Veterinary Clinical Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.

Original language	English (US)
Pages (from-to)	545-548
Number of pages	4
Journal	Research in veterinary science
Volume	94
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2013.01.007
State	Published - Jun 2013

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Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

Chemiluminescence
Feline
Immunocytochemistry
Tumor
Western Blot

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10.1016/j.rvsc.2013.01.007

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title = "Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks",

abstract = "Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.",

keywords = "Chemiluminescence, Feline, Immunocytochemistry, Tumor, Western Blot",

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T2 - An indicator of double strand DNA breaks

AU - Lawrence, J.

AU - Karpuzoglu, E.

AU - Vance, A.

AU - Vandenplas, M.

AU - Saba, C.

AU - Turek, M.

AU - Gogal, R. M.

PY - 2013/6

Y1 - 2013/6

N2 - Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.

AB - Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.

KW - Chemiluminescence

KW - Feline

KW - Immunocytochemistry

KW - Tumor

KW - Western Blot

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JO - Research in veterinary science

JF - Research in veterinary science

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ER -

Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks

Abstract

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