Cerebrospinal fluid glutamine, tryptophan, and tryptophan metabolite concentrations in dogs with portosystemic shunts

David E. Holt, Robert J. Washabau, Sina Djali, Betsy Dayrell-Hart, Kenneth J. Drobatz, Melvyn P. Heyes, Michael B. Robinson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective - To determine whether glutamine (GLN), tryptophan (TRP), and tryptophan metabolite concentrations are higher in cerebralspinal fluid (CSF) dogs with naturally occurring portosystemic shunts (PSS), compared with control dogs. Animals - 11 dogs with confirmed PSS and 12 control dogs fed low- and high-protein diets. Procedure - Cerebrospinal fluid and blood samples were collected from all dogs. Serum and CSF concentrations of GLN, alanine, serine, TRP, 5-hydroxyindoleacetic acid (5-HIAA), and quinolinic acid (QUIN) were measured. Results - Cerebrospinal fluid concentrations of GLN, TRP and 5-HIAA were significantly higher in PSS dogs, compared with control dogs fed high- or low-protein diets. Cerebrospinal fluid QUIN concentration was significantly higher in PSS dogs, compared with control dogs fed the low-protein diet. Serum QUIN concentration was significantly lower in PSS dogs, compared with control dogs fed either high- or low-protein diets. Conclusions and Clinical Relevance - An increase in CNS GLN concentration is associated with high CSF concentrations of TRP and TRP metabolites in dogs with PSS, High CSF 5-HIAA concentrations indicate an increased flux of TRP through the CNS serotonin metabolic pathway, whereas high CSF QUIN concentrations indicate an increased metabolism of TRP through the indolamine-2,3-dioxygenase pathway. The high CSF QUIN concentrations in the face of low serum QUIN concentrations in dogs with PSS indicates that QUIN production from TRP is occurring in the CNS. High concentrations of QUIN and other TRP metabolites in the CNS may contribute to neurologic abnormalities found in dogs with PSS and hepatic encephalopathy.

Original languageEnglish (US)
Pages (from-to)1167-1171
Number of pages5
JournalAmerican journal of veterinary research
Volume63
Issue number8
DOIs
StatePublished - Aug 1 2002

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Surgical Portasystemic Shunt
cerebrospinal fluid
Glutamine
glutamine
tryptophan
Cerebrospinal Fluid
Quinolinic Acid
Dogs
metabolites
dogs
Tryptophan
acids
5-hydroxyindoleacetic acid
Protein-Restricted Diet
Hydroxyindoleacetic Acid
low protein diet
blood serum
Hepatic Encephalopathy
tryptophyltryptophan
Proteins

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Cerebrospinal fluid glutamine, tryptophan, and tryptophan metabolite concentrations in dogs with portosystemic shunts. / Holt, David E.; Washabau, Robert J.; Djali, Sina; Dayrell-Hart, Betsy; Drobatz, Kenneth J.; Heyes, Melvyn P.; Robinson, Michael B.

In: American journal of veterinary research, Vol. 63, No. 8, 01.08.2002, p. 1167-1171.

Research output: Contribution to journalArticle

Holt, David E. ; Washabau, Robert J. ; Djali, Sina ; Dayrell-Hart, Betsy ; Drobatz, Kenneth J. ; Heyes, Melvyn P. ; Robinson, Michael B. / Cerebrospinal fluid glutamine, tryptophan, and tryptophan metabolite concentrations in dogs with portosystemic shunts. In: American journal of veterinary research. 2002 ; Vol. 63, No. 8. pp. 1167-1171.
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abstract = "Objective - To determine whether glutamine (GLN), tryptophan (TRP), and tryptophan metabolite concentrations are higher in cerebralspinal fluid (CSF) dogs with naturally occurring portosystemic shunts (PSS), compared with control dogs. Animals - 11 dogs with confirmed PSS and 12 control dogs fed low- and high-protein diets. Procedure - Cerebrospinal fluid and blood samples were collected from all dogs. Serum and CSF concentrations of GLN, alanine, serine, TRP, 5-hydroxyindoleacetic acid (5-HIAA), and quinolinic acid (QUIN) were measured. Results - Cerebrospinal fluid concentrations of GLN, TRP and 5-HIAA were significantly higher in PSS dogs, compared with control dogs fed high- or low-protein diets. Cerebrospinal fluid QUIN concentration was significantly higher in PSS dogs, compared with control dogs fed the low-protein diet. Serum QUIN concentration was significantly lower in PSS dogs, compared with control dogs fed either high- or low-protein diets. Conclusions and Clinical Relevance - An increase in CNS GLN concentration is associated with high CSF concentrations of TRP and TRP metabolites in dogs with PSS, High CSF 5-HIAA concentrations indicate an increased flux of TRP through the CNS serotonin metabolic pathway, whereas high CSF QUIN concentrations indicate an increased metabolism of TRP through the indolamine-2,3-dioxygenase pathway. The high CSF QUIN concentrations in the face of low serum QUIN concentrations in dogs with PSS indicates that QUIN production from TRP is occurring in the CNS. High concentrations of QUIN and other TRP metabolites in the CNS may contribute to neurologic abnormalities found in dogs with PSS and hepatic encephalopathy.",
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T1 - Cerebrospinal fluid glutamine, tryptophan, and tryptophan metabolite concentrations in dogs with portosystemic shunts

AU - Holt, David E.

AU - Washabau, Robert J.

AU - Djali, Sina

AU - Dayrell-Hart, Betsy

AU - Drobatz, Kenneth J.

AU - Heyes, Melvyn P.

AU - Robinson, Michael B.

PY - 2002/8/1

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N2 - Objective - To determine whether glutamine (GLN), tryptophan (TRP), and tryptophan metabolite concentrations are higher in cerebralspinal fluid (CSF) dogs with naturally occurring portosystemic shunts (PSS), compared with control dogs. Animals - 11 dogs with confirmed PSS and 12 control dogs fed low- and high-protein diets. Procedure - Cerebrospinal fluid and blood samples were collected from all dogs. Serum and CSF concentrations of GLN, alanine, serine, TRP, 5-hydroxyindoleacetic acid (5-HIAA), and quinolinic acid (QUIN) were measured. Results - Cerebrospinal fluid concentrations of GLN, TRP and 5-HIAA were significantly higher in PSS dogs, compared with control dogs fed high- or low-protein diets. Cerebrospinal fluid QUIN concentration was significantly higher in PSS dogs, compared with control dogs fed the low-protein diet. Serum QUIN concentration was significantly lower in PSS dogs, compared with control dogs fed either high- or low-protein diets. Conclusions and Clinical Relevance - An increase in CNS GLN concentration is associated with high CSF concentrations of TRP and TRP metabolites in dogs with PSS, High CSF 5-HIAA concentrations indicate an increased flux of TRP through the CNS serotonin metabolic pathway, whereas high CSF QUIN concentrations indicate an increased metabolism of TRP through the indolamine-2,3-dioxygenase pathway. The high CSF QUIN concentrations in the face of low serum QUIN concentrations in dogs with PSS indicates that QUIN production from TRP is occurring in the CNS. High concentrations of QUIN and other TRP metabolites in the CNS may contribute to neurologic abnormalities found in dogs with PSS and hepatic encephalopathy.

AB - Objective - To determine whether glutamine (GLN), tryptophan (TRP), and tryptophan metabolite concentrations are higher in cerebralspinal fluid (CSF) dogs with naturally occurring portosystemic shunts (PSS), compared with control dogs. Animals - 11 dogs with confirmed PSS and 12 control dogs fed low- and high-protein diets. Procedure - Cerebrospinal fluid and blood samples were collected from all dogs. Serum and CSF concentrations of GLN, alanine, serine, TRP, 5-hydroxyindoleacetic acid (5-HIAA), and quinolinic acid (QUIN) were measured. Results - Cerebrospinal fluid concentrations of GLN, TRP and 5-HIAA were significantly higher in PSS dogs, compared with control dogs fed high- or low-protein diets. Cerebrospinal fluid QUIN concentration was significantly higher in PSS dogs, compared with control dogs fed the low-protein diet. Serum QUIN concentration was significantly lower in PSS dogs, compared with control dogs fed either high- or low-protein diets. Conclusions and Clinical Relevance - An increase in CNS GLN concentration is associated with high CSF concentrations of TRP and TRP metabolites in dogs with PSS, High CSF 5-HIAA concentrations indicate an increased flux of TRP through the CNS serotonin metabolic pathway, whereas high CSF QUIN concentrations indicate an increased metabolism of TRP through the indolamine-2,3-dioxygenase pathway. The high CSF QUIN concentrations in the face of low serum QUIN concentrations in dogs with PSS indicates that QUIN production from TRP is occurring in the CNS. High concentrations of QUIN and other TRP metabolites in the CNS may contribute to neurologic abnormalities found in dogs with PSS and hepatic encephalopathy.

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