Cerebellar regional dissection for molecular analysis

Katherine A. Hamel, Marija Cvetanovic

Research output: Contribution to journalArticlepeer-review

Abstract

Cerebellum plays an important role in several key functions including control of movement, balance, cognition, reward, and affect. Imaging studies indicate that distinct cerebellar regions contribute to these different functions. Molecular studies examining regional cerebellar differences are lagging as they are mostly done on whole cerebellar extracts thereby masking any distinctions across specific cerebellar regions. Here we describe a technique to reproducibly and quickly dissect four different cerebellar regions: the deep cerebellar nuclei (DCN), anterior and posterior vermal cerebellar cortex, and the cerebellar cortex of the hemispheres. Dissecting out these distinct regions allows for the exploration of molecular mechanisms that may underlie their unique contributions to balance, movement, affect and cognition. This technique may also be used to explore differences in pathological susceptibility of these specific regions across various mouse disease models.

Original languageEnglish (US)
Article numbere61922
Pages (from-to)1-13
Number of pages13
JournalJournal of Visualized Experiments
Volume2020
Issue number166
DOIs
StatePublished - Dec 2020

Bibliographical note

Funding Information:
We are grateful to Austin Ferro and Juao-Guilherme Rosa in the Cvetanovic lab for their help in troubleshooting dissections and in RNA extraction and RTqPCR. This research is funded by M. Cvetanovic, R01 NS197387; HHS | National Institutes of Health (NIH).

Publisher Copyright:
© 2020 JoVE Journal of Visualized Experiments.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

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