Cellular Taxonomy of the Mouse Striatum as Revealed by Single-Cell RNA-Seq

Ozgun Gokce, Geoffrey M. Stanley, Barbara Treutlein, Norma F. Neff, J. Gray Camp, Robert C. Malenka, Patrick E. Rothwell, Marc V. Fuccillo, Thomas C. Südhof, Stephen R. Quake

Research output: Contribution to journalArticlepeer-review

265 Scopus citations

Abstract

The striatum contributes to many cognitive processes and disorders, but its cell types are incompletely characterized. We show that microfluidic and FACS-based single-cell RNA sequencing of mouse striatum provides a well-resolved classification of striatal cell type diversity. Transcriptome analysis revealed ten differentiated, distinct cell types, including neurons, astrocytes, oligodendrocytes, ependymal, immune, and vascular cells, and enabled the discovery of numerous marker genes. Furthermore, we identified two discrete subtypes of medium spiny neurons (MSNs) that have specific markers and that overexpress genes linked to cognitive disorders and addiction. We also describe continuous cellular identities, which increase heterogeneity within discrete cell types. Finally, we identified cell type-specific transcription and splicing factors that shape cellular identities by regulating splicing and expression patterns. Our findings suggest that functional diversity within a complex tissue arises from a small number of discrete cell types, which can exist in a continuous spectrum of functional states.

Original languageEnglish (US)
Pages (from-to)1126-1137
Number of pages12
JournalCell reports
Volume16
Issue number4
DOIs
StatePublished - Jul 26 2016

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© 2016 The Authors

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