Cellular Microbiaxial Stretching to Measure a Single-Cell Strain Energy Density Function

Zaw Win, Justin M. Buksa, Kerianne E. Steucke, G. W. Gant Luxton, Victor H Barocas, Patrick W Alford

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The stress in a cell due to extracellular mechanical stimulus is determined by its mechanical properties, and the structural organization of many adherent cells suggests that their properties are anisotropic. This anisotropy may significantly influence the cells' mechanotransductive response to complex loads, and has important implications for development of accurate models of tissue biomechanics. Standard methods for measuring cellular mechanics report linear moduli that cannot capture large-deformation anisotropic properties, which in a continuum mechanics framework are best described by a strain energy density function (SED). In tissues, the SED is most robustly measured using biaxial testing. Here, we describe a cellular microbiaxial stretching (ClBS) method that modifies this tissue-scale approach to measure the anisotropic elastic behavior of individual vascular smooth muscle cells (VSMCs) with nativelike cytoarchitecture. Using ClBS, we reveal that VSMCs are highly anisotropic under large deformations. We then characterize a Holzapfel-Gasser-Ogden type SED for individual VSMCs and find that architecture-dependent properties of the cells can be robustly described using a formulation solely based on the organization of their actin cytoskeleton. These results suggest that cellular anisotropy should be considered when developing biomechanical models, and could play an important role in cellular mechano-adaptation.

Original languageEnglish (US)
Article number071006
JournalJournal of Biomechanical Engineering
Volume139
Issue number7
DOIs
StatePublished - Jul 1 2017

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Strain energy
Probability density function
Stretching
Muscle
Vascular Smooth Muscle
Tissue
Smooth Muscle Myocytes
Anisotropy
Mechanics
Continuum mechanics
Biomechanics
Actin Cytoskeleton
Cells
Biomechanical Phenomena
Mechanical properties
Testing

Cite this

Cellular Microbiaxial Stretching to Measure a Single-Cell Strain Energy Density Function. / Win, Zaw; Buksa, Justin M.; Steucke, Kerianne E.; Gant Luxton, G. W.; Barocas, Victor H; Alford, Patrick W.

In: Journal of Biomechanical Engineering, Vol. 139, No. 7, 071006, 01.07.2017.

Research output: Contribution to journalArticle

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