Splenic adherent cells are required for generation of both primary and, at limiting antigen dose, secondary allogeneic responses of cytolytic T lymphocytes to intact stimulator cells. Secondary responses to purified allogeneic plasma membranes or purified H-2K(k) antigens in liposomes are also dependent upon splenic adherent cells. Generation of these responses requires the presence of an Ia-positive, Thy 1.2-negative, radiation-resistant cell in the splenic adherent cell population that is adherent to glass, plastic, and nylon wool. Stimulation of cytolytic T lymphocyte precursors with purified H-2K(k) alloantigen bound to Ia + splenic adherent cells is 10-20 times more efficient than stimulation with antigen added directly to culture. Furthermore, a marked decrease in the response of cytolytic T lymphocytes to liposomes was observed when antiserum against Ia(d) specific for the Ia of the responder cells was added to culture. These results demonstrate that, for purified proteins of the major histocompatibility complex, antigen presentation by Ia + splenic adherent cells plays a role in the generation of a cytolytic T lymphocyte response.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Issue number||10 II|
|State||Published - 1980|