Cellular applications of 31P and 13C nuclear magnetic resonance

R. G. Shulman, T. R. Brown, K. Ugurbil, S. Ogawa, S. M. Cohen, J. A. Den Hollander

Research output: Contribution to journalReview articlepeer-review

238 Scopus citations

Abstract

High-resolution nuclear magnetic resonance (NMR) studies of cells and purified mitochondria are discussed to show the kind of information that can be obtained in vivo. In suspensions of Escherichia coli both phosphorus-31 and carbon-13 NMR studies of glycolysis and bioenergetics are presented. In rat liver cells the pathways of gluconeogenesis from carbon-13-labeled glycerol are followed by carbon-13 NMR. In the intact liver cells cytosolic and mitochondrial pH's were separately measured by phosphorus-31 NMR. In purified mitochondria the internal and external concentrations of inorganic phosphate, adenosine diphosphate, and adenosine triphosphate were determined by phosphorus-31 NMR while the pH difference across the membrane was measured simultaneously.

Original languageEnglish (US)
Pages (from-to)160-166
Number of pages7
JournalScience
Volume205
Issue number4402
DOIs
StatePublished - 1979

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