Cell‐free Translation of Potyviral RNA Including Extracts from Disease Resistant Soybean

L. M. Mansky; D. P. Durand; J. H. Hill

doi:10.1111/j.1439-0434.1992.tb01300.x

Cell‐free Translation of Potyviral RNA Including Extracts from Disease Resistant Soybean

L. M. Mansky, D. P. Durand, J. H. Hill

Diagnostic and Biological Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cell‐free translation of soybean mosaic virus (SMV), strain G2 or G7, RNAs in the presence of fractionated leaf extracts from the cultivars Davis or Williams‘82 revealed no differences that were associated with disease resistance of the cultivars to the virus strains. Extracts of leaf tissue from Davis (resistant to strain G2) and susceptible Williams‘82 were fractionated into three broad peaks. Apparent proteinase activity was detected in peak II of both soybean cultivars in the cell‐free translation of SMV‐G2 or ‐G7 RNAs. Inhibition of translation occurred in the presence of peak extracts from either of the soybean cultivars at levels of 500 μ/ml and/or 1000 μ/ml of protein. No inhibition of proteolytic processing was observed by extracts from either cultivar.

Original language	English (US)
Pages (from-to)	211-220
Number of pages	10
Journal	Journal of Phytopathology
Volume	136
Issue number	3
DOIs	https://doi.org/10.1111/j.1439-0434.1992.tb01300.x
State	Published - Nov 1992

Publisher link

10.1111/j.1439-0434.1992.tb01300.x

Find It

Find It at U of M Libraries

Cite this

@article{7dfc71a4a06a4ebab52b635c2ac096ba,

title = "Cell‐free Translation of Potyviral RNA Including Extracts from Disease Resistant Soybean",

abstract = "Cell‐free translation of soybean mosaic virus (SMV), strain G2 or G7, RNAs in the presence of fractionated leaf extracts from the cultivars Davis or Williams{\textquoteleft}82 revealed no differences that were associated with disease resistance of the cultivars to the virus strains. Extracts of leaf tissue from Davis (resistant to strain G2) and susceptible Williams{\textquoteleft}82 were fractionated into three broad peaks. Apparent proteinase activity was detected in peak II of both soybean cultivars in the cell‐free translation of SMV‐G2 or ‐G7 RNAs. Inhibition of translation occurred in the presence of peak extracts from either of the soybean cultivars at levels of 500 μ/ml and/or 1000 μ/ml of protein. No inhibition of proteolytic processing was observed by extracts from either cultivar.",

author = "Mansky, {L. M.} and Durand, {D. P.} and Hill, {J. H.}",

year = "1992",

month = nov,

doi = "10.1111/j.1439-0434.1992.tb01300.x",

language = "English (US)",

volume = "136",

pages = "211--220",

journal = "Journal of Phytopathology",

issn = "0931-1785",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Cell‐free Translation of Potyviral RNA Including Extracts from Disease Resistant Soybean

AU - Mansky, L. M.

AU - Durand, D. P.

AU - Hill, J. H.

PY - 1992/11

Y1 - 1992/11

N2 - Cell‐free translation of soybean mosaic virus (SMV), strain G2 or G7, RNAs in the presence of fractionated leaf extracts from the cultivars Davis or Williams‘82 revealed no differences that were associated with disease resistance of the cultivars to the virus strains. Extracts of leaf tissue from Davis (resistant to strain G2) and susceptible Williams‘82 were fractionated into three broad peaks. Apparent proteinase activity was detected in peak II of both soybean cultivars in the cell‐free translation of SMV‐G2 or ‐G7 RNAs. Inhibition of translation occurred in the presence of peak extracts from either of the soybean cultivars at levels of 500 μ/ml and/or 1000 μ/ml of protein. No inhibition of proteolytic processing was observed by extracts from either cultivar.

AB - Cell‐free translation of soybean mosaic virus (SMV), strain G2 or G7, RNAs in the presence of fractionated leaf extracts from the cultivars Davis or Williams‘82 revealed no differences that were associated with disease resistance of the cultivars to the virus strains. Extracts of leaf tissue from Davis (resistant to strain G2) and susceptible Williams‘82 were fractionated into three broad peaks. Apparent proteinase activity was detected in peak II of both soybean cultivars in the cell‐free translation of SMV‐G2 or ‐G7 RNAs. Inhibition of translation occurred in the presence of peak extracts from either of the soybean cultivars at levels of 500 μ/ml and/or 1000 μ/ml of protein. No inhibition of proteolytic processing was observed by extracts from either cultivar.

UR - http://www.scopus.com/inward/record.url?scp=84936483904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84936483904&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0434.1992.tb01300.x

DO - 10.1111/j.1439-0434.1992.tb01300.x

M3 - Article

AN - SCOPUS:84936483904

SN - 0931-1785

VL - 136

SP - 211

EP - 220

JO - Journal of Phytopathology

JF - Journal of Phytopathology

IS - 3

ER -

Cell‐free Translation of Potyviral RNA Including Extracts from Disease Resistant Soybean

Abstract

Publisher link

Find It

Other files and links

Fingerprint

Cite this