Cell‐free Translation of Potyviral RNA Including Extracts from Disease Resistant Soybean

L. M. Mansky, D. P. Durand, J. H. Hill

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Cell‐free translation of soybean mosaic virus (SMV), strain G2 or G7, RNAs in the presence of fractionated leaf extracts from the cultivars Davis or Williams‘82 revealed no differences that were associated with disease resistance of the cultivars to the virus strains. Extracts of leaf tissue from Davis (resistant to strain G2) and susceptible Williams‘82 were fractionated into three broad peaks. Apparent proteinase activity was detected in peak II of both soybean cultivars in the cell‐free translation of SMV‐G2 or ‐G7 RNAs. Inhibition of translation occurred in the presence of peak extracts from either of the soybean cultivars at levels of 500 μ/ml and/or 1000 μ/ml of protein. No inhibition of proteolytic processing was observed by extracts from either cultivar.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalJournal of Phytopathology
Volume136
Issue number3
DOIs
StatePublished - Nov 1992

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