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Cell‐Free Desensitization of Opioid Inhibition of Adenylate Cyclase in Neuroblastoma × Glioma NG108–15 Hybrid Cell Membranes

  • Alan K. Louie
  • , Ping‐Yee ‐Y Law
  • , Horace H. Loh

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: When membranes from neuroblastoma |MX glioma NG108–15 hybrid cells were incubated in a cell‐free system with opioid agonists, a time‐, temperature‐, and dose‐dependent desensitization to opioid inhibition of adenylate cyclase activity was observed. The composition of the system during the incubation was manipulated to elucidate the biochemical mechanisms of desensitization. Receptor coupling appeared to be a prerequisite for desensitization, because both magnesium and sodium, which are necessary for coupling, were required for desensitization. Removal of ATP and addition of cyclic AMP or cyclic GMP had no effect on desensitization.

Original languageEnglish (US)
Pages (from-to)733-737
Number of pages5
JournalJournal of Neurochemistry
Volume47
Issue number3
DOIs
StatePublished - Sep 1986
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Adenylate cyclase
  • Desensitization
  • Neuroblastoma |MX glioma hybrid cells
  • Opioid

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