Cell‐Free Desensitization of Opioid Inhibition of Adenylate Cyclase in Neuroblastoma × Glioma NG108–15 Hybrid Cell Membranes

Alan K. Louie; Ping‐Yee ‐Y Law; Horace H. Loh

doi:10.1111/j.1471-4159.1986.tb00673.x

Cell‐Free Desensitization of Opioid Inhibition of Adenylate Cyclase in Neuroblastoma × Glioma NG108–15 Hybrid Cell Membranes

Alan K. Louie, Ping‐Yee ‐Y Law, Horace H. Loh

Research output: Contribution to journal › Article › peer-review

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Abstract

Abstract: When membranes from neuroblastoma |MX glioma NG108–15 hybrid cells were incubated in a cell‐free system with opioid agonists, a time‐, temperature‐, and dose‐dependent desensitization to opioid inhibition of adenylate cyclase activity was observed. The composition of the system during the incubation was manipulated to elucidate the biochemical mechanisms of desensitization. Receptor coupling appeared to be a prerequisite for desensitization, because both magnesium and sodium, which are necessary for coupling, were required for desensitization. Removal of ATP and addition of cyclic AMP or cyclic GMP had no effect on desensitization.

Original language	English (US)
Pages (from-to)	733-737
Number of pages	5
Journal	Journal of Neurochemistry
Volume	47
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-4159.1986.tb00673.x
State	Published - Sep 1986
Externally published	Yes

Keywords

Adenylate cyclase
Desensitization
Neuroblastoma |MX glioma hybrid cells
Opioid

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1471-4159.1986.tb00673.x

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title = "Cell‐Free Desensitization of Opioid Inhibition of Adenylate Cyclase in Neuroblastoma × Glioma NG108–15 Hybrid Cell Membranes",

abstract = "Abstract: When membranes from neuroblastoma |MX glioma NG108–15 hybrid cells were incubated in a cell‐free system with opioid agonists, a time‐, temperature‐, and dose‐dependent desensitization to opioid inhibition of adenylate cyclase activity was observed. The composition of the system during the incubation was manipulated to elucidate the biochemical mechanisms of desensitization. Receptor coupling appeared to be a prerequisite for desensitization, because both magnesium and sodium, which are necessary for coupling, were required for desensitization. Removal of ATP and addition of cyclic AMP or cyclic GMP had no effect on desensitization.",

keywords = "Adenylate cyclase, Desensitization, Neuroblastoma |MX glioma hybrid cells, Opioid",

author = "Louie, {Alan K.} and Law, {Ping‐Yee ‐Y} and Loh, {Horace H.}",

year = "1986",

month = sep,

doi = "10.1111/j.1471-4159.1986.tb00673.x",

language = "English (US)",

volume = "47",

pages = "733--737",

journal = "Journal of Neurochemistry",

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TY - JOUR

T1 - Cell‐Free Desensitization of Opioid Inhibition of Adenylate Cyclase in Neuroblastoma × Glioma NG108–15 Hybrid Cell Membranes

AU - Louie, Alan K.

AU - Law, Ping‐Yee ‐Y

AU - Loh, Horace H.

PY - 1986/9

Y1 - 1986/9

N2 - Abstract: When membranes from neuroblastoma |MX glioma NG108–15 hybrid cells were incubated in a cell‐free system with opioid agonists, a time‐, temperature‐, and dose‐dependent desensitization to opioid inhibition of adenylate cyclase activity was observed. The composition of the system during the incubation was manipulated to elucidate the biochemical mechanisms of desensitization. Receptor coupling appeared to be a prerequisite for desensitization, because both magnesium and sodium, which are necessary for coupling, were required for desensitization. Removal of ATP and addition of cyclic AMP or cyclic GMP had no effect on desensitization.

AB - Abstract: When membranes from neuroblastoma |MX glioma NG108–15 hybrid cells were incubated in a cell‐free system with opioid agonists, a time‐, temperature‐, and dose‐dependent desensitization to opioid inhibition of adenylate cyclase activity was observed. The composition of the system during the incubation was manipulated to elucidate the biochemical mechanisms of desensitization. Receptor coupling appeared to be a prerequisite for desensitization, because both magnesium and sodium, which are necessary for coupling, were required for desensitization. Removal of ATP and addition of cyclic AMP or cyclic GMP had no effect on desensitization.

KW - Adenylate cyclase

KW - Desensitization

KW - Neuroblastoma |MX glioma hybrid cells

KW - Opioid

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U2 - 10.1111/j.1471-4159.1986.tb00673.x

DO - 10.1111/j.1471-4159.1986.tb00673.x

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C2 - 3016185

AN - SCOPUS:0022485677

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Cell‐Free Desensitization of Opioid Inhibition of Adenylate Cyclase in Neuroblastoma × Glioma NG108–15 Hybrid Cell Membranes

Abstract

Keywords

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