Cell penetrating peptide conjugated chitosan for enhanced delivery of nucleic acid

Buddhadev Layek, Lindsey Lipp, Jagdish Singh

Research output: Contribution to journalReview articlepeer-review

54 Scopus citations


Gene therapy is an emerging therapeutic strategy for the cure or treatment of a spectrum of genetic disorders. Nevertheless, advances in gene therapy are immensely reliant upon design of an efficient gene carrier that can deliver genetic cargoes into the desired cell populations. Among various nonviral gene delivery systems, chitosan-based carriers have gained increasing attention because of their high cationic charge density, excellent biocompatibility, nearly nonexistent cytotoxicity, negligible immune response, and ideal ability to undergo chemical conjugation. However, a major shortcoming of chitosan-based carriers is their poor cellular uptake, leading to inadequate transfection efficiency. The intrinsic feature of cell penetrating peptides (CPPs) for transporting diverse cargoes into multiple cell and tissue types in a safe manner suggests that they can be conjugated to chitosan for improving its transfection efficiency. In this review, we briefly discuss CPPs and their classification, and also the major mechanisms contributing to the cellular uptake of CPPs and cargo conjugates. We also discuss immense improvements for the delivery of nucleic acids using CPP-conjugated chitosan-based carriers with special emphasis on plasmid DNA and small interfering RNA.

Original languageEnglish (US)
Pages (from-to)28912-28930
Number of pages19
JournalInternational journal of molecular sciences
Issue number12
StatePublished - Dec 4 2015

Bibliographical note

Publisher Copyright:
© 2015 by the authors; licensee MDPI, Basel, Switzerland.


  • Cell penetrating peptides (CPPs)
  • Cellular uptake
  • Chitosan
  • Gene delivery
  • Nonviral vector
  • Transfection


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