Cell line development for biomanufacturing processes: recent advances and an outlook

Huong Le, Nandita Vishwanathan, Nitya M. Jacob, Mugdha Gadgil, Wei Shou Hu

Research output: Contribution to journalReview articlepeer-review

20 Scopus citations

Abstract

At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances in the past decade, from vector design to cell line screening, have greatly expanded our capability to attain producing cell lines with certain desired traits. Increasing availability of genomic and transcriptomic resources for industrially important cell lines coupled with advances in genome editing technology have opened new avenues for cell line development. These developments are poised to help biosimilar manufacturing, which requires targeting pre-defined product quality attributes, e.g., glycoform, to match the innovator’s range. This review summarizes recent advances and discusses future possibilities in this area.

Original languageEnglish (US)
Pages (from-to)1553-1564
Number of pages12
JournalBiotechnology Letters
Volume37
Issue number8
DOIs
StatePublished - Aug 1 2015

Keywords

  • Biomanufacturing
  • Biosimilars
  • CHO cells genomics
  • Cell line development
  • Genome editing
  • Transcriptomics

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