Cell line development for biomanufacturing processes

Mugdha Gadgil, Wei Shou Hu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A large number of recombinant protein therapeutics like monoclonal antibodies are manufactured in animal cells because of their ability to provide appropriate post-translational modifications. Protein production using animal cells requires the development of a cell line with the gene of interest integrated into the host cell genome and stably expressing the protein of interest at an acceptable level of productivity. This chapter provides a summary of the various steps required for the development of such a cell line starting from the transfection of the gene of interest into an appropriate host cell. The identification of such a cell line currently requires the screening of a large number of single-cell clones subsequent to transfection to identify an acceptable clone. However, recent advances in molecular approaches and high-throughput screening techniques, summarized here in brief, are now allowing an increase in the success rate of identifying a suitable cell line.

Original languageEnglish (US)
Title of host publicationApplied Bioengineering
Subtitle of host publicationInnovations and Future Directions
PublisherWiley
Pages355-382
Number of pages28
ISBN (Electronic)9783527800582
ISBN (Print)9783527340750
DOIs
StatePublished - Jan 1 2017

Bibliographical note

Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA.

Keywords

  • Animal cell culture for protein expression
  • Cell line development
  • Clone screening
  • Protein expression
  • Protein production

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