Abstract
A large number of recombinant protein therapeutics like monoclonal antibodies are manufactured in animal cells because of their ability to provide appropriate post-translational modifications. Protein production using animal cells requires the development of a cell line with the gene of interest integrated into the host cell genome and stably expressing the protein of interest at an acceptable level of productivity. This chapter provides a summary of the various steps required for the development of such a cell line starting from the transfection of the gene of interest into an appropriate host cell. The identification of such a cell line currently requires the screening of a large number of single-cell clones subsequent to transfection to identify an acceptable clone. However, recent advances in molecular approaches and high-throughput screening techniques, summarized here in brief, are now allowing an increase in the success rate of identifying a suitable cell line.
Original language | English (US) |
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Title of host publication | Applied Bioengineering |
Subtitle of host publication | Innovations and Future Directions |
Publisher | Wiley |
Pages | 355-382 |
Number of pages | 28 |
ISBN (Electronic) | 9783527800582 |
ISBN (Print) | 9783527340750 |
DOIs | |
State | Published - Jan 1 2017 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2017 Wiley-VCH Verlag GmbH & Co. KGaA.
Keywords
- Animal cell culture for protein expression
- Cell line development
- Clone screening
- Protein expression
- Protein production