Abstract
Precision medicine requires high throughput cell isolation and measurement that maintains physiology. Unfortunately, many techniques are slow or alter cell biomarkers cells. This necessitates new approaches, which we achieve by integrating affinity-based cell isolation with spiral microfluidics. We characterize the device via computational simulations, predicting wall shear stress within an order of magnitude of arterial wall shear stress (~0.2 Pa). We identify that poly-l-lysine supplementation preserves cell geometry and improves cell release. We demonstrate preservation of angiogenic biomarker concentrations, measuring 1,000–2,000 vascular endothelial growth factor receptor-1 per human umbilical vein endothelial cell, which is in line with the previously reported measurements. We attain 76.7 ± 9.0% release of captured cells by integrating thermophoresis and optimizing buffer residence time. Ultimately, we find that combining affinity-based cell isolation (secondary anchor targeted cell release) with spiral microfluidics offers a fast, biomarker preserving approach needed to individualize medicine.
| Original language | English (US) |
|---|---|
| Article number | e16844 |
| Journal | AIChE Journal |
| Volume | 65 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2019 |
Bibliographical note
Funding Information:The authors would like to thank the following foundations for their funding support: AHA: 16SDG26940002; NSF CAREER: 1653925; NSF CBET: 1512598; NSF BPE: 1648454; NSF: 1743333; NSF: 1743334; and NSF: 1640783. The authors would also like to thank Dr Grosman, for his assistance with measuring osmolarities in his lab, as well as the Fredrick Seitz Material Research Laboratory for their assistance with Atomic Force Microscopy. Additionally, the authors would like to thank the Imoukhuede Lab members such as Dr Jared C. Weddell, Stacie Chen, Spencer Mamer, Cheri Fang, and Colin Castleberry, as well as the expertise of Wendy Woods, Ashley Oyirifi, Jiaojiao Wang, and Pierrick Gallerne for intriguing and educational conversations and assistance. Also, the authors would like to personally thank Jad Maamari, Allen Bell III, Lucas Lasher, Daphne Shen, Shweta Bhushan, Thomas Ninan, Kathleen Ferreira, and Ivan Villamar for their assistance with material preparation and experimental assistance.
Funding Information:
The authors would like to thank the following foundations for their funding support: AHA: 16SDG26940002; NSF CAREER: 1653925; NSF CBET: 1512598; NSF BPE: 1648454; NSF: 1743333; NSF: 1743334; and NSF: 1640783. The authors would also like to thank Dr Grosman, for his assistance with measuring osmolarities in his lab, as well as the Fredrick Seitz Material Research Laboratory for their assistance with Atomic Force Microscopy. Additionally, the authors would like to thank the Imoukhuede Lab members such as Dr Jared C. Weddell, Stacie Chen, Spencer Mamer, Cheri Fang, and Colin Castleberry, as well as the expertise of Wendy Woods, Ashley Oyirifi, Jiaojiao Wang, and Pierrick Gallerne for intriguing and educational conversations and assistance. Also, the authors would like to personally thank Jad Maamari, Allen Bell III, Lucas Lasher, Daphne Shen, Shweta Bhushan, Thomas Ninan, Kathleen Ferreira, and Ivan Villamar for their assistance with material preparation and experimental assistance.
Publisher Copyright:
© 2019 American Institute of Chemical Engineers
Keywords
- cell isolation
- microfluidics
- precision medicine
- surface chemistry/physics
- thermophoresis