Cell-free translation in lysates from Spodoptera frugiperda (Lepidoptera: Noctuidae) cells

Mavis R. Swerdel, Ann M Fallon

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

1. 1. Conditions for in vitro translation of mRNA in cell-free extracts from cultured Spodoptera frugiperda cells were defined. 2. 2. Incorporation of [35S]methionine into acid-precipitable material increased for approximately 1 hr, and was sensitive to the protein synthesis inhibitors pactamycin and cycloheximide. 3. 3. Micrococcal nuclease-treated lysate, primed with purified rabbit globin mRNA, synthesized a major protein with the size of full length globin, indicating that the lysate supported correct initiation and elongation of polypeptides.

Original languageEnglish (US)
Pages (from-to)803-806
Number of pages4
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume93
Issue number4
DOIs
StatePublished - 1989

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