Cell-free transcription–translation: engineering biology from the nanometer to the millimeter scale

David Garenne, Vincent Noireaux

Research output: Contribution to journalReview article

Abstract

Cell-free transcription–translation (TXTL) has become a highly versatile technology to construct, characterize and interrogate genetically programmed biomolecular systems implemented outside living organisms. By recapitulating gene expression in vitro, TXTL offers unparalleled flexibility to take apart, engineer and analyze quantitatively the effects of chemical, physical and genetic contexts on the function of biochemical systems, from simple regulatory elements to millimeter-scale pattern formation. Here, we review the capabilities of the current cell-free platforms for executing DNA programs in vitro. We describe the recent advances in programming using cell-free expression, a multidisciplinary playground that has enabled a myriad of novel applications in synthetic biology, biotechnology, and biological physics. Finally, we discuss the challenges and perspectives in the research area of TXTL-based constructive biology.

LanguageEnglish (US)
Pages19-27
Number of pages9
JournalCurrent opinion in biotechnology
Volume58
DOIs
StatePublished - Aug 1 2019

Fingerprint

Transcription
Biotechnology
Gene expression
DNA
Physics
Engineers
Synthetic Biology
Technology
Gene Expression
Research
In Vitro Techniques

Cite this

Cell-free transcription–translation : engineering biology from the nanometer to the millimeter scale. / Garenne, David; Noireaux, Vincent.

In: Current opinion in biotechnology, Vol. 58, 01.08.2019, p. 19-27.

Research output: Contribution to journalReview article

@article{bb43be248ac149bd91dab72fcdbd66c4,
title = "Cell-free transcription–translation: engineering biology from the nanometer to the millimeter scale",
abstract = "Cell-free transcription–translation (TXTL) has become a highly versatile technology to construct, characterize and interrogate genetically programmed biomolecular systems implemented outside living organisms. By recapitulating gene expression in vitro, TXTL offers unparalleled flexibility to take apart, engineer and analyze quantitatively the effects of chemical, physical and genetic contexts on the function of biochemical systems, from simple regulatory elements to millimeter-scale pattern formation. Here, we review the capabilities of the current cell-free platforms for executing DNA programs in vitro. We describe the recent advances in programming using cell-free expression, a multidisciplinary playground that has enabled a myriad of novel applications in synthetic biology, biotechnology, and biological physics. Finally, we discuss the challenges and perspectives in the research area of TXTL-based constructive biology.",
author = "David Garenne and Vincent Noireaux",
year = "2019",
month = "8",
day = "1",
doi = "10.1016/j.copbio.2018.10.007",
language = "English (US)",
volume = "58",
pages = "19--27",
journal = "Current Opinion in Biotechnology",
issn = "0958-1669",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Cell-free transcription–translation

T2 - Current Opinion in Biotechnology

AU - Garenne, David

AU - Noireaux, Vincent

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Cell-free transcription–translation (TXTL) has become a highly versatile technology to construct, characterize and interrogate genetically programmed biomolecular systems implemented outside living organisms. By recapitulating gene expression in vitro, TXTL offers unparalleled flexibility to take apart, engineer and analyze quantitatively the effects of chemical, physical and genetic contexts on the function of biochemical systems, from simple regulatory elements to millimeter-scale pattern formation. Here, we review the capabilities of the current cell-free platforms for executing DNA programs in vitro. We describe the recent advances in programming using cell-free expression, a multidisciplinary playground that has enabled a myriad of novel applications in synthetic biology, biotechnology, and biological physics. Finally, we discuss the challenges and perspectives in the research area of TXTL-based constructive biology.

AB - Cell-free transcription–translation (TXTL) has become a highly versatile technology to construct, characterize and interrogate genetically programmed biomolecular systems implemented outside living organisms. By recapitulating gene expression in vitro, TXTL offers unparalleled flexibility to take apart, engineer and analyze quantitatively the effects of chemical, physical and genetic contexts on the function of biochemical systems, from simple regulatory elements to millimeter-scale pattern formation. Here, we review the capabilities of the current cell-free platforms for executing DNA programs in vitro. We describe the recent advances in programming using cell-free expression, a multidisciplinary playground that has enabled a myriad of novel applications in synthetic biology, biotechnology, and biological physics. Finally, we discuss the challenges and perspectives in the research area of TXTL-based constructive biology.

UR - http://www.scopus.com/inward/record.url?scp=85055827870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055827870&partnerID=8YFLogxK

U2 - 10.1016/j.copbio.2018.10.007

DO - 10.1016/j.copbio.2018.10.007

M3 - Review article

VL - 58

SP - 19

EP - 27

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

SN - 0958-1669

ER -