Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1

John A. McFarlane; David Garenne; Vincent Noireaux; Steven D. Bowden

doi:10.1016/j.mimet.2024.106920

Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1

John A. McFarlane, David Garenne, Vincent Noireaux, Steven D. Bowden

Research output: Contribution to journal › Article › peer-review

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Abstract

Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage felixO1 using an E. coli-based CFE system.

Original language	English (US)
Article number	106920
Journal	Journal of Microbiological Methods
Volume	220
DOIs	https://doi.org/10.1016/j.mimet.2024.106920
State	Published - May 2024

Bibliographical note

Publisher Copyright:
© 2023

Keywords

Cell-free expression
Phage
Salmonella
felixO1

PubMed: MeSH publication types

Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mimet.2024.106920

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abstract = "Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage felixO1 using an E. coli-based CFE system.",

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AU - McFarlane, John A.

AU - Garenne, David

AU - Noireaux, Vincent

AU - Bowden, Steven D.

PY - 2024/5

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AB - Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage felixO1 using an E. coli-based CFE system.

KW - Cell-free expression

KW - Phage

KW - Salmonella

KW - felixO1

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ER -

Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

UN SDGs

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