Cell-free expression with the toxic amino acid canavanine

Emanuel G. Worst, Matthias P. Exner, Alessandro De Simone, Marc Schenkelberger, Vincent Noireaux, Nediljko Budisa, Albrecht Ott

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Abstract Canavanine is a naturally occurring noncanonical amino acid, which is analogous to arginine. It is a potent antimetabolite and natural allelochemic agent, capable of affecting or blocking regulatory and catalytic reactions that involve arginine. Incorporated into proteins at arginine positions, canavanine can be detrimental to protein stability and functional integrity. Although incorporation of canavanine into proteins has long been documented, due to its toxicity, expression in Escherichia coli and other common hosts remains a considerable challenge. Here, we present a simple, cell-free expression system with markedly improved performance compared to heterologous expression. The cell-free expression system does not require any tuning besides substitution of arginine by canavanine. We show that our technique enables highly efficient protein expression in small volumes with arginine being fully replaced by canavanine for functional and structural studies.

Original languageEnglish (US)
Article number22835
Pages (from-to)3658-3660
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume25
Issue number17
DOIs
StatePublished - Aug 3 2015

Bibliographical note

Funding Information:
M.P.E. and N.B. acknowledge financial support from the FP7 EU -funded METACODE and SYNPEPTIDE Consortia. E.G.W., M.S. and A.O. acknowledge financial support by the Deutsche Forschungsgemeinschaft (DFG) within the collaborative research center SFB 1027.

Keywords

  • Antimetabolite
  • Arginine
  • Canavanine
  • Cell-free expression
  • Noncanonical amino acid

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