Cell cycle regulation of condensin Smc4

Hsu Wei-Shan, Vas C. Amit, Duncan J Clarke

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The condensin complex is a conserved ATPase which promotes the compaction of chromatin during mitosis in eukaryotic cells. Condensin complexes have in addition been reported to contribute to interphase processes including sister chromatid cohesion. It is not understood how condensins specifically become competent to facilitate chromosome condensation in preparation for chromosome segregation in anaphase. Here we describe evidence that core condensin subunits are regulated at the level of protein stability in budding yeast. In particular, Smc2 and Smc4 abundance is cell cycle regulated, peaking at mitosis and falling to low levels in interphase. Smc4 degradation at the end of mitosis is dependent on the Anaphase Promoting Complex/Cyclosome and is mediated by the proteasome. Overproduction of Smc4 results in delayed decondensation, but has a limited ability to promote premature condensation in interphase. Unexpectedly, the Mad2 spindle checkpoint protein is required for mitotic Smc4 degradation. These studies have revealed the novel finding that condensin protein levels are cell cycle regulated and have identified the factors necessary for Smc4 proteolysis.

Original languageEnglish (US)
Pages (from-to)263-276
Number of pages14
JournalOncotarget
Volume10
Issue number3
DOIs
StatePublished - Jan 1 2019

Bibliographical note

Funding Information:
condensin transcript profiles. This work was supported by

Funding Information:
We thank Steve Haase for help with the analysis of condensin transcript profiles. This work was supported by NSF grant MCB-0842157 and NIH grant R01GM112793 (DJC).

Publisher Copyright:
Copyright Wei-Shan et al.

Keywords

  • Anaphase promoting complex
  • Condensin
  • Mad2
  • Smc2
  • Smc4

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