Rap1 proteins are capable of competing with Ras p21 for binding to effectors, and of antagonizing some Ras-induced effects, but their participation in normal growth regulation has not been established. The level of Rap1 protein and the expression of the rap1A gene were examined by immunoblotting and Northern analysis during the regenerative growth response in rat liver following partial hepatectomy. Protein and mRNA were significantly down-regulated prior to and during the onset of DNA synthesis. The timing of this effect is consistent with a model in which expression of Rap1 is turned off or down to allow the initiation of proliferation.
|Number of pages
|Biochemical and Biophysical Research Communications
|Published - Jan 23 1997
Bibliographical noteFunding Information:
The authors wish to thank Nikos Katopodis and Danielle Perry for technical assistance and Gary Bokoch and Larry Quilliam for Rap1 antiserum and cDNA. This work was supported by a National Science Foundation Presidential Young Investigator Award.