Cell-based protein sensor array using genetically engineered microbes

Sang Hyun Oh, Sang Ho Lee, Sophia A. Kenrick, Patrick S. Daugherty, H. T. Soh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A cell-based protein-detecting array was developed on which genetically engineered microbes were spatially organized as sensor elements in a microfluidic device. Whole E. coli cells displaying protein-binding peptide ligands at high density on their outer membrane served as effective capture reagents. Cells were immobilized on gold electrodes using dielectrophoresis, thus allowing each sensor element to be electrically addressable. On-chip fluorescence analysis and flow cytometry were used to confirm highly specific capture of target molecules. The use of dielectrophoresis in microfluidic devices enabled efficient utilization of whole-cell capture reagents for a variety of protein detection and diagnostic applications.

Original languageEnglish (US)
Title of host publication19th IEEE International Conference on Micro Electro Mechanical Systems
Pages486-489
Number of pages4
StatePublished - Oct 24 2006
Event19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey
Duration: Jan 22 2006Jan 26 2006

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2006
ISSN (Print)1084-6999

Other

Other19th IEEE International Conference on Micro Electro Mechanical Systems
CountryTurkey
CityIstanbul
Period1/22/061/26/06

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