TY - JOUR
T1 - CD94 defines phenotypically and functionally distinct mouse NK cell subsets
AU - Yu, Jianhua
AU - Wei, Min
AU - Mao, Hsiaoyin
AU - Zhang, Jianying
AU - Hughes, Tiffany
AU - Mitsui, Takeki
AU - Park, Il Kyoo
AU - Hwang, Christine
AU - Liu, Shujun
AU - Marcucci, Guido
AU - Trotta, Rossana
AU - Benson, Don M.
AU - Caligiuri, Michael A.
PY - 2009/10/15
Y1 - 2009/10/15
N2 - Understanding of heterogeneous NK subsets is important for the study of NK cell biology and development, and for the application of NK cell-based therapies in the treatment of disease. Here we demonstrate that the surface expression of CD94 can distinctively divide mouse NK cells into two approximately even CD94low and CD94high subsets in all tested organs and tissues. The CD94high NK subset has significantly greater capacity to proliferate, produce IFN-γ, and lyse target cells than does the CD94 low subset. The CD94high subset has exclusive expression of NKG2A/C/E, higher expression of CD117 and CD69, and lower expression of Ly49D (activating) and Ly49G2 (inhibitory). In vivo, purified mouse CD94 low NK cells become CD94high NK cells, but not vice versa. Collectively, our data suggest that CD94 is an Ag that can be used to identify functionally distinct NK cell subsets in mice and could also be relevant to late-stage mouse NK cell development.
AB - Understanding of heterogeneous NK subsets is important for the study of NK cell biology and development, and for the application of NK cell-based therapies in the treatment of disease. Here we demonstrate that the surface expression of CD94 can distinctively divide mouse NK cells into two approximately even CD94low and CD94high subsets in all tested organs and tissues. The CD94high NK subset has significantly greater capacity to proliferate, produce IFN-γ, and lyse target cells than does the CD94 low subset. The CD94high subset has exclusive expression of NKG2A/C/E, higher expression of CD117 and CD69, and lower expression of Ly49D (activating) and Ly49G2 (inhibitory). In vivo, purified mouse CD94 low NK cells become CD94high NK cells, but not vice versa. Collectively, our data suggest that CD94 is an Ag that can be used to identify functionally distinct NK cell subsets in mice and could also be relevant to late-stage mouse NK cell development.
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U2 - 10.4049/jimmunol.0900907
DO - 10.4049/jimmunol.0900907
M3 - Article
C2 - 19801519
AN - SCOPUS:77951734903
SN - 0022-1767
VL - 183
SP - 4968
EP - 4974
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -