CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation

Mark A. Daniels, Lesley Devine, Joseph D. Miller, Janice M. Moser, Aron E. Lukacher, John D. Altman, Paula Kavathas, Kristin A Hogquist, Stephen C Jameson

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Abstract

CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8+ T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.

Original languageEnglish (US)
Pages (from-to)1051-1061
Number of pages11
JournalImmunity
Volume15
Issue number6
DOIs
StatePublished - Jan 1 2001

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    Daniels, M. A., Devine, L., Miller, J. D., Moser, J. M., Lukacher, A. E., Altman, J. D., Kavathas, P., Hogquist, K. A., & Jameson, S. C. (2001). CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation. Immunity, 15(6), 1051-1061. https://doi.org/10.1016/S1074-7613(01)00252-7