CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils

Keith M Skubitz, Kenneth D. Campbell, Amy P Skubitz

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Four members of the carcinoembryonic antigen family, CD66a, CD66b, CD66c, and CD66d, are expressed on human neutrophils. In neutrophils these proteins are activation antigens in that their surface expression is increased following stimulation. To examine their potential role in neutrophil signaling, the effects on neutrophil adhesion to human umbilical vein endothelial cells of a panel of well characterized CD66 mAbs was tested, CD66a, CD66b, CD66c, and CD66d antibodies each increased neutrophil adhesion to human umbilical vein endothelial cell monolayers. This increase in neutrophil adhesion caused by CD66 antibodies was blocked by a CD18 antibody and associated with up-regalation of CD11/CD18 on the neutrophil surface. This increase in neutrophil adhesion required physiological extracellular calcium concentrations at or near the time of CD66 antibody binding to the neutrophil. The incubation of CD66 antibodies with neutrophils in the absence of calcium for 10 min before repletion of calcium resulted in no increase in neutrophil adhesion. The data suggest that CD66, CD66b, CD66c, and CD66d antibody binding to the neutrophil surface triggers a transient activation signal that requires extracellular calcium and regulates the adhesive activity of CD11/CD18. Sequential desensitization experiments indicated that CD66a, CD66b, CD66c, and CD66d can each independently transmit signals in neutrophils.

Original languageEnglish (US)
Pages (from-to)106-117
Number of pages12
JournalJournal of Leukocyte Biology
Volume60
Issue number1
DOIs
StatePublished - Jul 1996

Keywords

  • Biliary glycoprotein
  • CD66
  • Carcinoembryonic antigen
  • Cell adhesion
  • Endothelial cells
  • Monoclonal antibodies

Fingerprint

Dive into the research topics of 'CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils'. Together they form a unique fingerprint.

Cite this