CD56 dim CD57+ NKG2C+ NK cell expansion is associated with reduced leukemia relapse after reduced intensity HCT

F. Cichocki, S. Cooley, Z. Davis, T. E. DeFor, H. Schlums, B. Zhang, C. G. Brunstein, B. R. Blazar, J. Wagner, D. J. Diamond, M. R. Verneris, Y. T. Bryceson, D. J. Weisdorf, J. S. Miller

Research output: Contribution to journalArticlepeer-review

164 Scopus citations


We have recently described a specialized subset of human natural killer (NK) cells with a CD56 dim CD57 + NKG2C + phenotype that expand specifically in response to cytomegalovirus (CMV) reactivation in hematopoietic cell transplant (HCT) recipients and exhibit properties characteristic of adaptive immunity. We hypothesize that these cells mediate relapse protection and improve post-HCT outcomes. In 674 allogeneic HCT recipients, we found that those who reactivated CMV had lower leukemia relapse (26% (17-35%), P=0.05) and superior disease-free survival (DFS) (55% (45-65%) P=0.04) 1 year after reduced intensity conditioning (RIC) compared with CMV seronegative recipients who experienced higher relapse rates (35% (27-43%)) and lower DFS (46% (38-54%)). This protective effect was independent of age and graft-vs-host disease and was not observed in recipients who received myeloablative regimens. Analysis of the reconstituting NK cells demonstrated that CMV reactivation is associated with both higher frequencies and greater absolute numbers of CD56 dim CD57 + NKG2C + NK cells, particularly after RIC HCT. Furthermore, expansion of these cells at 6 months posttransplant independently trended toward a lower 2-year relapse risk. Together, our data suggest that the protective effect of CMV reactivation on posttransplant relapse is in part driven by adaptive NK cell responses.

Original languageEnglish (US)
Pages (from-to)456-463
Number of pages8
Issue number2
StatePublished - Feb 1 2016

Bibliographical note

Funding Information:
We thank the following shared resources within the Masonic Cancer Center at the University of Minnesota: The Translational Therapy Laboratory, Flow Cytometry Shared Resource, Clinical Trials Office, Oncology Medical Informatics and Services, and the Biostatistical Core. This work was supported by NIH P30 CA77598, R01 CA72669 (to BRB), 1R01 CA181045 (to DJD), 5R01 CA077544 (to DJD) and P30 CA033572 (City of Hope Cancer Center). FC is an Amy Strelzer Manasevit Research Program Scholar and is supported by a National Marrow Donor Program Award (CON000000052310).

Publisher Copyright:
© 2016 Macmillan Publishers Limited.


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