CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis

Matthew D. Martin, Cara Skon-Hegg, Caleb Y. Kim, Julie Xu, Tamara A. Kucaba, Whitney Swanson, Mark J. Pierson, Jesse W. Williams, Vladimir P. Badovinac, Steven S. Shen, Molly A. Ingersoll, Thomas S. Griffith

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Uropathogenic E. coli (UPEC) is a primary organism responsible for urinary tract infections and a common cause of sepsis. Microbially experienced laboratory mice, generated by cohousing with pet store mice, exhibit increased morbidity and mortality to polymicrobial sepsis or lipopolysaccharide challenge. By contrast, cohoused mice display significant resistance, compared with specific pathogen-free mice, to a monomicrobial sepsis model using UPEC. CD115+ monocytes mediate protection in the cohoused mice, as depletion of these cells leads to increased mortality and UPEC pathogen burden. Further study of the cohoused mice reveals increased TNF-α production by monocytes, a skewing toward Ly6ChiCD115+ “classical” monocytes, and enhanced egress of Ly6ChiCD115+ monocytes from the bone marrow. Analysis of cohoused bone marrow also finds increased frequency and number of myeloid multipotent progenitor cells. These results show that a history of microbial exposure impacts innate immunity in mice, which can have important implications for the preclinical study of sepsis.

Original languageEnglish (US)
Article number113345
JournalCell reports
Issue number11
StatePublished - Nov 28 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)


  • CD115
  • CP: Immunology
  • CP: Microbiology
  • dirty mice
  • monocyte
  • myelopoiesis
  • sepsis


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