Cathepsin E is a specific marker of dysplasia in APC Min/+ mouse intestine

Lizette Busquets, Hector Guillen, Melanie E. DeFord, Mark A. Suckow, Rudolph M. Navari, Francis J. Castellino, Mary Prorok

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Transcriptional profiling of APC Min/+ mouse intestinal epithelial tissue has revealed that cathepsin E (catE) manifests high relative expression in adenomas and carcinomas relative to normal epithelium. Real-time RT-PCR data presented previously confirm the presence of catE transcript in APC Min/+ adenomatous cells compared with samples derived from normal APC Min/+ and wild-type tissue. At the protein level, strong, highly specific immunohistochemical staining for catE is displayed in dysplastic lesions of APC Min/+ mice. Using Western immunoblot analyses, it was additionally established that the urine of tumor-bearing mice contains higher levels of the monomeric form of catE than their wild-type counterparts. These results authenticate the relationship between transcript abundance and protein levels in transformed tissue and suggest potential utility for catE as a marker for the inception and progression of intestinal cancers.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalTumor Biology
Volume27
Issue number1
DOIs
StatePublished - Dec 1 2005

Keywords

  • APC mice
  • Cathepsin E
  • Immunohistochemistry
  • Intestinal adenomas

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