TY - JOUR
T1 - Catch and Release DNA Decoys
T2 - Capture and Photochemical Dissociation of NF-κB Transcription Factors
AU - Struntz, Nicholas B.
AU - Harki, Daniel A.
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/6/17
Y1 - 2016/6/17
N2 - Catch and release DNA decoys (CRDDs) are a new class of non-natural DNA probes that capture and dissociate from DNA-binding proteins using a light trigger. Photolytic cleavage of non-natural nucleobases in the CRDD yields abasic sites and truncation products that lower the affinity of the CRDD for its protein target. Herein, we demonstrate the ability of the first-generation CRDD to bind and release NF-κB proteins. This platform technology should be applicable to other DNA-binding proteins by modification of the target sequence.
AB - Catch and release DNA decoys (CRDDs) are a new class of non-natural DNA probes that capture and dissociate from DNA-binding proteins using a light trigger. Photolytic cleavage of non-natural nucleobases in the CRDD yields abasic sites and truncation products that lower the affinity of the CRDD for its protein target. Herein, we demonstrate the ability of the first-generation CRDD to bind and release NF-κB proteins. This platform technology should be applicable to other DNA-binding proteins by modification of the target sequence.
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U2 - 10.1021/acschembio.6b00130
DO - 10.1021/acschembio.6b00130
M3 - Article
C2 - 27054264
AN - SCOPUS:84975318620
SN - 1554-8929
VL - 11
SP - 1631
EP - 1638
JO - ACS Chemical Biology
JF - ACS Chemical Biology
IS - 6
ER -