Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus

John S. Albin; William L. Brown; Reuben S. Harris

doi:10.1016/j.virol.2013.11.041

Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus

John S. Albin, William L. Brown, Reuben S. Harris

Molecular Biology (BMBB)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

APOBEC3 proteins are DNA cytosine deaminases that restrict the replication of human immunodeficiency virus deficient in the counterdefense protein Vif. Here, we address the capacity of APOBEC3F to restrict via deaminase-dependent and -independent mechanisms by monitoring spreading infections in diverse T cell lines. Our data indicate that only a deaminase-proficient protein is capable of long-term restriction of Vif-deficient HIV in T cells, analogous to prior reports for APOBEC3G. This indicates that the principal mechanism of APOBEC3F restriction is deaminase-dependent.

Original language	English (US)
Pages (from-to)	49-54
Number of pages	6
Journal	Virology
Volume	450-451
DOIs	https://doi.org/10.1016/j.virol.2013.11.041
State	Published - Feb 2014

Bibliographical note

Funding Information:
We thank Judd Hultquist, Brett Anderson, and Leah Evans for thoughtful comments. This research was funded by the National Institute of Allergy and Infectious Diseases ( R01 AI064046 to RSH) and by the National Institute of General Medical Sciences ( P01 GM091743 to RSH). JSA was supported in part by the National Institute on Drug Abuse ( F30 DA026310 ) and by the University of Minnesota Medical Scientist Training Program ( T32 GM008244 ).

Keywords

APOBEC3F
APOBEC3G
Deaminase
HIV restriction
Vif

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2013.11.041

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title = "Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus",

abstract = "APOBEC3 proteins are DNA cytosine deaminases that restrict the replication of human immunodeficiency virus deficient in the counterdefense protein Vif. Here, we address the capacity of APOBEC3F to restrict via deaminase-dependent and -independent mechanisms by monitoring spreading infections in diverse T cell lines. Our data indicate that only a deaminase-proficient protein is capable of long-term restriction of Vif-deficient HIV in T cells, analogous to prior reports for APOBEC3G. This indicates that the principal mechanism of APOBEC3F restriction is deaminase-dependent.",

keywords = "APOBEC3F, APOBEC3G, Deaminase, HIV restriction, Vif",

author = "Albin, {John S.} and Brown, {William L.} and Harris, {Reuben S.}",

note = "Funding Information: We thank Judd Hultquist, Brett Anderson, and Leah Evans for thoughtful comments. This research was funded by the National Institute of Allergy and Infectious Diseases ( R01 AI064046 to RSH) and by the National Institute of General Medical Sciences ( P01 GM091743 to RSH). JSA was supported in part by the National Institute on Drug Abuse ( F30 DA026310 ) and by the University of Minnesota Medical Scientist Training Program ( T32 GM008244 ).",

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doi = "10.1016/j.virol.2013.11.041",

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journal = "Virology",

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T1 - Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus

AU - Albin, John S.

AU - Brown, William L.

AU - Harris, Reuben S.

N1 - Funding Information: We thank Judd Hultquist, Brett Anderson, and Leah Evans for thoughtful comments. This research was funded by the National Institute of Allergy and Infectious Diseases ( R01 AI064046 to RSH) and by the National Institute of General Medical Sciences ( P01 GM091743 to RSH). JSA was supported in part by the National Institute on Drug Abuse ( F30 DA026310 ) and by the University of Minnesota Medical Scientist Training Program ( T32 GM008244 ).

PY - 2014/2

Y1 - 2014/2

N2 - APOBEC3 proteins are DNA cytosine deaminases that restrict the replication of human immunodeficiency virus deficient in the counterdefense protein Vif. Here, we address the capacity of APOBEC3F to restrict via deaminase-dependent and -independent mechanisms by monitoring spreading infections in diverse T cell lines. Our data indicate that only a deaminase-proficient protein is capable of long-term restriction of Vif-deficient HIV in T cells, analogous to prior reports for APOBEC3G. This indicates that the principal mechanism of APOBEC3F restriction is deaminase-dependent.

AB - APOBEC3 proteins are DNA cytosine deaminases that restrict the replication of human immunodeficiency virus deficient in the counterdefense protein Vif. Here, we address the capacity of APOBEC3F to restrict via deaminase-dependent and -independent mechanisms by monitoring spreading infections in diverse T cell lines. Our data indicate that only a deaminase-proficient protein is capable of long-term restriction of Vif-deficient HIV in T cells, analogous to prior reports for APOBEC3G. This indicates that the principal mechanism of APOBEC3F restriction is deaminase-dependent.

KW - APOBEC3F

KW - APOBEC3G

KW - Deaminase

KW - HIV restriction

KW - Vif

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DO - 10.1016/j.virol.2013.11.041

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SN - 0042-6822

VL - 450-451

SP - 49

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JO - Virology

JF - Virology

ER -

Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus

Abstract

Bibliographical note

Keywords

UN SDGs

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