APOBEC3 proteins are DNA cytosine deaminases that restrict the replication of human immunodeficiency virus deficient in the counterdefense protein Vif. Here, we address the capacity of APOBEC3F to restrict via deaminase-dependent and -independent mechanisms by monitoring spreading infections in diverse T cell lines. Our data indicate that only a deaminase-proficient protein is capable of long-term restriction of Vif-deficient HIV in T cells, analogous to prior reports for APOBEC3G. This indicates that the principal mechanism of APOBEC3F restriction is deaminase-dependent.
Bibliographical noteFunding Information:
We thank Judd Hultquist, Brett Anderson, and Leah Evans for thoughtful comments. This research was funded by the National Institute of Allergy and Infectious Diseases ( R01 AI064046 to RSH) and by the National Institute of General Medical Sciences ( P01 GM091743 to RSH). JSA was supported in part by the National Institute on Drug Abuse ( F30 DA026310 ) and by the University of Minnesota Medical Scientist Training Program ( T32 GM008244 ).
- HIV restriction