Carrier of wingless (Cow) regulation of drosophila neuromuscular junction development

Danielle L. Kopke, Shannon N. Leahy, Dominic J. Vita, Sofia C. Lima, Zachary L. Newman, Kendal Broadie

Research output: Contribution to journalArticlepeer-review

Abstract

The first Wnt signaling ligand discovered, Drosophila Wingless [Wg (Wnt1 in mammals)], plays critical roles in neuromuscular junction (NMJ) development, regulating synaptic architecture, and function. Heparan sulfate pro-teoglycans (HSPGs), consisting of a core protein with heparan sulfate (HS) glycosaminoglycan (GAG) chains, bind to Wg ligands to control both extracellular distribution and intercellular signaling function. Drosophila HSPGs previously shown to regulate Wg trans-synaptic signaling at the NMJ include the glypican Dally-like protein (Dlp) and perlecan Terribly Reduced Optic Lobes (Trol). Here, we investigate synaptogenic functions of the most recently described Drosophila HSPG, secreted Carrier of Wingless (Cow), which directly binds Wg in the extracellular space. At the glutamatergic NMJ, we find that Cow secreted from the presynaptic motor neuron acts to limit synaptic architecture and neurotransmission strength. In cow null mutants, we find increased synaptic bouton number and elevated excitatory current amplitudes, phenocopying presynaptic Wg overexpression. We show cow null mutants exhibit an increased number of glutamatergic synapses and increased synaptic vesicle fusion frequency based both on GCaMP imaging and electrophysiology recording. We find that membrane-tethered Wg prevents cow null defects in NMJ development, indicating that Cow mediates secreted Wg signal-ing. It was shown previously that the secreted Wg deacylase Notum restricts Wg signaling at the NMJ, and we show here that Cow and Notum work through the same pathway to limit synaptic development. We conclude Cow acts cooperatively with Notum to coordinate neuromuscular synapse structural and functional differentiation via negative regulation of Wg trans-synaptic signaling within the extracellular synaptomatrix.

Original languageEnglish (US)
Article numberENEURO.0285-19.2020
JournaleNeuro
Volume7
Issue number2
DOIs
StatePublished - 2020
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health Grants MH-096832 to K.B. and F31-MH-111144 to D.L.K. Correspondence should be addressed to Kendal Broadie at kendal. broadie@vanderbilt.edu. https://doi.org/10.1523/ENEURO.0285-19.2020 Copyright © 2020 Kopke et al.

Publisher Copyright:
© 2020 Kopke et al.

Keywords

  • Drosophila
  • HSPG
  • Neuromuscular junction
  • Synaptomatrix

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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