Abstract
Cachexia is a muscle wasting syndrome occurring in many advanced cancer patients. Cachexia significantly increases cancer morbidity and mortality. Cardiac atrophy and contractility deficits have been observed in patients and in animal models with cancer cachexia, which may contribute to cachexia pathophysiology. However, underlying contributors to decreased in vivo cardiac contractility are not well understood. In this study, we sought to distinguish heart-intrinsic changes from systemic factors contributing to cachexia-associated cardiac dysfunction. We hypothesized that isolated heart and cardiac myocyte functional deficits underlie in vivo contractile dysfunction. To test this hypothesis, isolated heart and cardiac myocyte function was measured in the colon-26 adenocarcinoma murine model of cachexia. Ex vivo perfused hearts from cachectic animals exhibited marked contraction and relaxation deficits during basal and pacing conditions. Isolated myocytes displayed significantly decreased peak contraction and relaxation rates, which was accompanied by decreased peak calcium and decay rates. This study uncovers significant organ and cellular-level functional deficits in cachectic hearts outside of the catabolic in vivo environment, which is explained in part by impaired calcium cycling. These data provide insight into physiological mechanisms of cardiomyopathy in cachexia, which is critical for the ultimate development of effective treatments for patients.
Original language | English (US) |
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Article number | 23627 |
Journal | Scientific reports |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - Dec 2021 |
Bibliographical note
Funding Information:This work was supported in part by funds from NIH HL132874 (JMM). We thank Dr. Frazer Heinis for training and assistance in Langendorff experiments. In memoriam, we thank our colleague, Dr. Joshua Martindale, for his advice, support, and mentorship.
Publisher Copyright:
© 2021, The Author(s).
PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural