Cardiac Fibroblast-Derived 3D Extracellular Matrix Seeded with Mesenchymal Stem Cells as a Novel Device to Transfer Cells to the Ischemic Myocardium

Eric G. Schmuck, Jacob D. Mulligan, Rebecca L. Ertel, Nicholas A. Kouris, Brenda M. Ogle, Amish N. Raval, Kurt W. Saupe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Demonstrate a novel manufacturing method to generate extracellular matrix scaffolds from cardiac fibroblasts (CF-ECM) as a therapeutic mesenchymal stem cell-transfer device. Rat CF were cultured at high-density (~1.6 × 105/cm2) for 10-14 days. Cell sheets were removed from the culture dish by incubation with EDTA and decellularized with water and peracetic acid. CF-ECM was characterized by mass spectrometry, immunofluorescence and scanning electron microscopy. CF-ECM seeded with human embryonic stem cell derived mesenchymal stromal cells (hEMSCs) were transferred into a mouse myocardial infarction model. 48 h later, mouse hearts were excised and examined for CF-ECM scaffold retention and cell transfer. CF-ECM scaffolds are composed of fibronectin (82%), collagens type I (13%), type III (3.4%), type V (0.2%), type II (0.1%) elastin (1.3%) and 18 non-structural bioactive molecules. Scaffolds remained intact on the mouse heart for 48 h without the use of sutures or glue. Identified hEMSCs were distributed from the epicardium to the endocardium. High density cardiac fibroblast culture can be used to generate CF-ECM scaffolds. CF-ECM scaffolds seeded with hEMSCs can be maintained on the heart without suture or glue. hEMSC are successfully delivered throughout the myocardium.

Original languageEnglish (US)
Pages (from-to)119-131
Number of pages13
JournalCardiovascular Engineering and Technology
Volume5
Issue number1
DOIs
StatePublished - Jan 1 2014

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Military electronic countermeasures
Fibroblasts
Stem cells
Mesenchymal Stromal Cells
Extracellular Matrix
Myocardium
Collagen Type XIII
Equipment and Supplies
Adhesives
Sutures
Scaffolds
Peracetic Acid
Endocardium
Elastin
Pericardium
Glues
Fibronectins
Edetic Acid
Electron Scanning Microscopy
Scaffolds (biology)

Keywords

  • Cardiac
  • Cardiac fibroblast
  • Extracellular matrix
  • Heart failure
  • Myocardial infarction
  • Regeneration
  • Stem cell

Cite this

Cardiac Fibroblast-Derived 3D Extracellular Matrix Seeded with Mesenchymal Stem Cells as a Novel Device to Transfer Cells to the Ischemic Myocardium. / Schmuck, Eric G.; Mulligan, Jacob D.; Ertel, Rebecca L.; Kouris, Nicholas A.; Ogle, Brenda M.; Raval, Amish N.; Saupe, Kurt W.

In: Cardiovascular Engineering and Technology, Vol. 5, No. 1, 01.01.2014, p. 119-131.

Research output: Contribution to journalArticle

Schmuck, Eric G. ; Mulligan, Jacob D. ; Ertel, Rebecca L. ; Kouris, Nicholas A. ; Ogle, Brenda M. ; Raval, Amish N. ; Saupe, Kurt W. / Cardiac Fibroblast-Derived 3D Extracellular Matrix Seeded with Mesenchymal Stem Cells as a Novel Device to Transfer Cells to the Ischemic Myocardium. In: Cardiovascular Engineering and Technology. 2014 ; Vol. 5, No. 1. pp. 119-131.
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