Abstract
Human induced pluripotent stem cell cardiomyocytes (hiPSC-CMs) are based on ground-breaking technology that has significantly impacted cardiovascular research. They provide a renewable source of human cardiomyocytes for a variety of applications including in vitro disease modeling and drug toxicity testing. Cardiac calcium regulation plays a critical role in the cardiomyocyte and is often dysregulated in cardiovascular disease. Due to the limited availability of human cardiac tissue, calcium handling and its regulation have most commonly been studied in the context of animal models. hiPSC-CMs can provide unique insights into human physiology and pathophysiology, although a remaining limitation is the relative immaturity of these cells compared to adult cardiomyocytes Therefore, this field is rapidly developing techniques to improve the maturity of hiPSC-CMs, further establishing their place in cardiovascular research. This review briefly covers the basics of cardiomyocyte calcium cycling and hiPSC technology, and will provide a detailed description of our current understanding of calcium in hiPSC-CMs.
Original language | English (US) |
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Article number | 986107 |
Journal | Frontiers in Cell and Developmental Biology |
Volume | 10 |
DOIs | |
State | Published - Jan 18 2023 |
Bibliographical note
Funding Information:This work was supported by a Doris Duke Charitable Foundation Clinical Scientist Development Award 2020103A (FK) and NIH T32 HL144472 (PE).
Publisher Copyright:
Copyright © 2023 Ernst, Bidwell, Dora, Thomas and Kamdar.
Keywords
- calcium
- cardiomyocytes
- disease modeling
- genetic cardiovascular diseases
- human induced pluripotent stem cells
- maturation
- stem cells
PubMed: MeSH publication types
- Journal Article
- Review