Carbonylated Proteins and Their Metabolic Regulation: Overview of Mechanisms, Target Proteins, and Characterization Using Proteomic Methods

Somaieh Afiuni-Zadeh, Timothy J. Griffin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Protein carbonylation is commonly accepted as biomarker of oxidative stress and is associated with a long list of pathological states and disorders. A detailed look at obesity-driven protein carbonylation illustrates the complex interplay between metabolic regulation of reactive carbonyls and other factors promoting oxidative stress and reactive oxygen species (ROS) production. Carbonylated proteins are detected using biotin-directed probes such as avidin coupled to a fluorescent reporter, which produces a carbonylation blot that may be used for general assessment of protein modification. A number of groups have described what can be termed "multifunctional" reagents for derivatizing and analyzing carbonylated proteins by mass spectrometry (MS). The multifunctional reagent possesses a number of capabilities for protein carbonylation characterization, including detection via immunoblotting, enrichment of labeled carbonylated proteins from complex mixtures, and identification and quantification of carbonyl-modified peptides using the tandem mass tag (TMT)-based functionality of the tag.

Original languageEnglish (US)
Title of host publicationProtein Carbonylation
Subtitle of host publicationPrinciples, Analysis, and Biological Implications
PublisherWiley
Pages110-130
Number of pages21
ISBN (Electronic)9781119374947
ISBN (Print)9781119074915
DOIs
StatePublished - Apr 20 2017

Keywords

  • Carbonylated protein
  • Metabolic regulation
  • Multifunctional reagents
  • Oxidative stress
  • Reactive carbonyls
  • Reactive oxygen species production

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