Carbon nanotube thin film pH electrode for potentiometric enzymatic acetylcholine biosensing

Dongjin Lee, Tianhong Cui

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Carboxylated single-walled carbon nanotube thin-film (SWCNT-TF) is demonstrated as a pH electrode and a platform for enzymatic potentiometric acetylcholine biosensors. SWCNT is layer-by-layer assembled with a positively charged polyelectrolyte on the metal electrode. The open circuit potential on SWCNT-TF electrode is measured with reference to an Ag/AgCl electrode in various pH buffers. With the aid of acetylcholinesterase (AchE), acetylcholine (Ach) is successfully detected using the SWCNT-TF pH electrode that demonstrates Nernst potential of -43 mV/pH. AchE-modified electrode has a sensing resolution of 10 μM of Ach and sensitivity of 19 mV/decade. The SWCNT-TF pH electrode fabricated by layer-by-layer self-assembly may play an important role in micro/nanoscale electrochemical sensors.

Original languageEnglish (US)
Pages (from-to)39-42
Number of pages4
JournalMicroelectronic Engineering
StatePublished - May 2012

Bibliographical note

Funding Information:
The authors gratefully acknowledge the support of NSF Grant No. CMMI 0735268 . Parts of this work were carried out in the Characterization Facility and the Nanofabrication Center, University of Minnesota, which are partially supported by NSF.


  • Acetylcholine sensor
  • Carbon nanotube
  • Electrochemical biosensor
  • Thin film
  • pH sensor


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