Carbachol-regulation of the expression of αi1-subunit of Gi and inositol-[l,4,5]-trisphosphate receptor in brain samples obtained from neonatal and adult rats

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Abstract

The basal steady-state concentration and the transcription rate of αGi1-mRNA in neonatal brain were greater than those in adult brain. The T 1 2 values for αGi1-mRNA in neonatal and adult samples did not differ significantly. This suggests that the resting concentration of αGi1-mRNA is regulated by its synthesis in brain. The basal steady-state concentration, the transcription rate and the t 1 2 values of [l,4,5]IP3R-mRNA in neonatal and adult brains did not differ significantly. Carbachol increased the concentration and the transcription rate of αGi1-mRNA and [1,4,5]IP3R-mRNA both in neonatal and adult samples. The increase in αGi1-mRNA concentration was affected by the age of the animals but the increase in [l,4,5]IP3R-mRNA concentration was not. However, the increase in the TR of αGi1-mRNA or [l,4,5]IP3R-mRNA was affected by the age of the animals. In the presence of carbachol, the degradation of mRNA occurred in two phases: a fast-phase with t 1 2 ranging from 1.4 to 1.8 h and a slow phase with t 1 2 ranging from 4 to 7 h. The t 1 2 values were not affected by the age of the animals. This study, therefore, suggests that developmental process down-regulates the expression of αGi1-mRNA but does not affect the expression of [l,4,5]IP3R-mRNA in brain. Carbachol increases the steady-state concentration of both αGi1-mRNA and [l,4,5]IP3R-mRNA possibly by altering their synthesis and stability in brain.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalComparative Biochemistry and Physiology. Part C: Comparative
Volume112
Issue number2
DOIs
StatePublished - Oct 1995

Keywords

  • Adult brain
  • G
  • Neonatal brain
  • Stability
  • Transcription rate
  • [1,4,5]IPR
  • mRNA

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