Capillary electrophoresis for the investigation of prostate-specific antigen heterogeneity

Maura J. Donohue, Mary B. Satterfield, Joseph J. Dalluge, Michael J. Welch, James E. Girard, David M. Bunk

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Prostate-specific antigen (PSA) is a single-chain glycoprotein that is used as a biomarker for prostate-related diseases. PSA has one known posttranslational modification, a sialylated diantennary N-linked oligosaccharide attached to the asparagine residue N45. In this study capillary electrophoresis (CE) was employed to separate the isoforms of seven commercially available free PSA samples, two of which were specialized: enzymatically active PSA and noncomplexing PSA. The free PSA samples examined migrated as four to nine distinct, highly resolved peaks, indicating the presence of several isoforms differing in their oligosaccharide compositions. Overall, the use of CE provides a rapid, reproducible method for separation of PSA into its individual isoforms.

Original languageEnglish (US)
Pages (from-to)318-327
Number of pages10
JournalAnalytical Biochemistry
Volume339
Issue number2
DOIs
StatePublished - Apr 15 2005
Externally publishedYes

Bibliographical note

Funding Information:
MJD thanks the National Science Foundation for the Chemometrics Fellowship and the National Institute of Standards and Technology for their support of this research.

Keywords

  • Capillary electrophoresis
  • Heterogeneity
  • Isoforms
  • Prostate-specific antigen

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