Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine

Alison S. Laufer; Jonathan C. Thomas; Marisol Figueira; Janneane F. Gent; Stephen I. Pelton; Melinda M. Pettigrew

doi:10.1016/j.vaccine.2009.12.078

Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine

Alison S. Laufer, Jonathan C. Thomas, Marisol Figueira, Janneane F. Gent, Stephen I. Pelton, Melinda M. Pettigrew

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Abstract

Non-vaccine Streptococcus pneumoniae serotypes are increasingly associated with disease. We evaluated isolates of the same sequence type (ST199) but different serotypes (15B/C, 19A) for growth in vitro, and pathogenic potential in a chinchilla otitis media model. We also developed a quantitative PCR (qPCR) assay to quantitatively assess each isolate, circumventing the need for selectable markers. In vitro studies showed faster growth of serotype 19A over 15B/C. Both were equally capable of colonization and middle ear infection in this model. Serotype 19A is included in new conjugate vaccine formulations while serotype 15B/C is not. Non-capsular vaccine targets will be important in disease prevention efforts.

Original language	English (US)
Pages (from-to)	2450-2457
Number of pages	8
Journal	Vaccine
Volume	28
Issue number	12
DOIs	https://doi.org/10.1016/j.vaccine.2009.12.078
State	Published - Mar 11 2010
Externally published	Yes

Keywords

Conjugate vaccine
Streptococcus pneumoniae
qPCR assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2009.12.078

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title = "Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine",

abstract = "Non-vaccine Streptococcus pneumoniae serotypes are increasingly associated with disease. We evaluated isolates of the same sequence type (ST199) but different serotypes (15B/C, 19A) for growth in vitro, and pathogenic potential in a chinchilla otitis media model. We also developed a quantitative PCR (qPCR) assay to quantitatively assess each isolate, circumventing the need for selectable markers. In vitro studies showed faster growth of serotype 19A over 15B/C. Both were equally capable of colonization and middle ear infection in this model. Serotype 19A is included in new conjugate vaccine formulations while serotype 15B/C is not. Non-capsular vaccine targets will be important in disease prevention efforts.",

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T1 - Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media

T2 - Implications for the conjugate vaccine

AU - Laufer, Alison S.

AU - Thomas, Jonathan C.

AU - Figueira, Marisol

AU - Gent, Janneane F.

AU - Pelton, Stephen I.

AU - Pettigrew, Melinda M.

PY - 2010/3/11

Y1 - 2010/3/11

N2 - Non-vaccine Streptococcus pneumoniae serotypes are increasingly associated with disease. We evaluated isolates of the same sequence type (ST199) but different serotypes (15B/C, 19A) for growth in vitro, and pathogenic potential in a chinchilla otitis media model. We also developed a quantitative PCR (qPCR) assay to quantitatively assess each isolate, circumventing the need for selectable markers. In vitro studies showed faster growth of serotype 19A over 15B/C. Both were equally capable of colonization and middle ear infection in this model. Serotype 19A is included in new conjugate vaccine formulations while serotype 15B/C is not. Non-capsular vaccine targets will be important in disease prevention efforts.

AB - Non-vaccine Streptococcus pneumoniae serotypes are increasingly associated with disease. We evaluated isolates of the same sequence type (ST199) but different serotypes (15B/C, 19A) for growth in vitro, and pathogenic potential in a chinchilla otitis media model. We also developed a quantitative PCR (qPCR) assay to quantitatively assess each isolate, circumventing the need for selectable markers. In vitro studies showed faster growth of serotype 19A over 15B/C. Both were equally capable of colonization and middle ear infection in this model. Serotype 19A is included in new conjugate vaccine formulations while serotype 15B/C is not. Non-capsular vaccine targets will be important in disease prevention efforts.

KW - Conjugate vaccine

KW - Streptococcus pneumoniae

KW - qPCR assay

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Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine

Abstract

Keywords

UN SDGs

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