Canopy fluorescence sensing for in-season maize nitrogen status diagnosis

Rui Dong, Yuxin Miao, Xinbing Wang, Fei Yuan, Krzysztof Kusnierek

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Accurate assessment of crop nitrogen (N) status and understanding the N demand are considered essential in precision N management. Chlorophyll fluorescence is unsusceptible to confounding signals from underlying bare soil and is closely related to plant photosynthetic activity. Therefore, fluorescence sensing is considered a promising technology for monitoring crop N status, even at an early growth stage. The objectives of this study were to evaluate the potential of using Multiplex® 3, a proximal canopy fluorescence sensor, to detect N status variability and to quantitatively estimate N status indicators at four key growth stages of maize. The sensor measurements were performed at different growth stages, and three different regression methods were compared to estimate plant N concentration (PNC), plant N uptake (PNU), and N nutrition index (NNI). The results indicated that the induced differences in maize plant N status were detectable as early as the V6 growth stage. The first method based on simple regression (SR) and the Multiplex sensor indices normalized by growing degree days (GDD) or N sufficiency index (NSI) achieved acceptable estimation accuracy (R2 = 0.73–0.87), showing a good potential of canopy fluorescence sensing for N status estimation. The second method using multiple linear regression (MLR), fluorescence indices and GDDs had the lowest modeling accuracy (R2 = 0.46–0.79). The third tested method used a non-linear regression approach in the form of random forest regression (RFR) based on multiple sensor indices and GDDs. This approach achieved the best estimation accuracy (R2 = 0.84–0.93) and the most accurate diagnostic result.

Original languageEnglish (US)
Article number5141
JournalRemote Sensing
Issue number24
StatePublished - Dec 1 2021

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.


  • Fluorescence sensing
  • Machine learning
  • Multiple linear regression
  • Nitrogen status
  • Precision nitrogen management


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