Candidate resistance genes to foliar phylloxera identified at Rdv3 of hybrid grape

Lu Yin, Avinash Karn, Lance Cadle-Davidson, Cheng Zou, Jason Londo, Qi Sun, Matthew D. Clark

Research output: Contribution to journalArticlepeer-review

Abstract

The foliage of the native grape species Vitis riparia and certain cold-hardy hybrid grapes are particularly susceptible to the insect pest phylloxera, Daktulosphaira vitifoliae Fitch. A previous study using a cold-hardy hybrid grape biparental F1 population (N ~ 125) detected the first quantitative trait locus (QTL) for foliar resistance on chromosome 14, designated as resistance to Daktulosphaira vitifoliae 3 (Rdv3). This locus spans a ~ 7-Mbp (10-20 cM) region and is too wide for effective marker-assisted selection or identification of candidate genes. Therefore, we fine mapped the QTL using a larger F1 population, GE1783 (N ~ 1023), and genome-wide rhAmpSeq haplotype markers. Through three selective phenotyping experiments replicated in the greenhouse, we screened 184 potential recombinants of GE1783 using a 0 to 7 severity rating scale among other phylloxera severity traits. A 500-kb fine mapped region at 4.8 Mbp on chromosome 14 was identified. The tightly linked rhAmpSeq marker 14_4 805 213 and flanking markers can be used for future marker-assisted breeding. This region contains 36 candidate genes with predicted functions in disease resistance (R genes and Bonzai genes) and gall formation (bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase). Disease resistance genes suggest a traditional R-gene-mediated resistance mechanism often accompanied by a hypersensitive response, which has been widely studied in the plant pathology field. A novel resistance mechanism, non-responsiveness to phylloxera gall formation is proposed as a function of the bifunctional dehydratase gene, which plays a role in gallic acid biosynthesis and is important in gall formation. This study has implications for improvement of foliar phylloxera resistance in cold-hardy hybrid germplasm and is a starting place to understand the mechanism of resistance in crops to gall-forming insects.

Original languageEnglish (US)
Article numberuhac027
JournalHorticulture Research
Volume9
DOIs
StatePublished - 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s). Published by Oxford University Press on behalf of Nanjing Agricultural University.

Keywords

  • Candidate genes
  • Fine mapping
  • Foliar phylloxera resistance
  • Gall-forming insect
  • Genetic mapping
  • Hybrid grape

PubMed: MeSH publication types

  • Journal Article

Fingerprint

Dive into the research topics of 'Candidate resistance genes to foliar phylloxera identified at Rdv3 of hybrid grape'. Together they form a unique fingerprint.

Cite this