Candida species differ in their interactions with immature human gastrointestinal epithelial cells

Christina Falgier, Sara Kegley, Heather A Podgorski, Timothy Heisel, Kathleen Storey, Catherine M Bendel, Cheryl A Gale

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Life-threatening gastrointestinal (GI) diseases of prematurity are highly associated with systemic candidiasis. This implicates the premature GI tract as an important site for invasion by Candida. Invasive interactions of Candida spp. with immature enterocytes have heretofore not been analyzed. Using a primary immature human enterocyte line, we compared the ability of multiple isolates of different Candida spp. to penetrate, injure, and induce a cytokine response from host cells. Of all the Candida spp. analyzed, C. albicans had the greatest ability to penetrate and injure immature enterocytes and to elicit IL-8 release (p < 0.01). In addition, C. albicans was the only Candida spp. to form filamentous hyphae when in contact with immature enterocytes. Similarly, a C. albicans mutant with defective hyphal morphogenesis and invasiveness had attenuated cytotoxicity for immature enterocytes (p < 0.003). Thus, hyphal morphogenesis correlates with immature enterocyte penetration, injury, and inflammatory responses. Furthermore, variability in enterocyte injury was observed among hyphal-producing C. albicans strains, suggesting that individual organism genotypes also influence host-pathogen interactions. Overall, the finding that Candida spp. differed in their interactions with immature enterocytes implicates that individual spp. may use different pathogenesis mechanisms.

Original languageEnglish (US)
Pages (from-to)384-389
Number of pages6
JournalPediatric Research
Volume69
Issue number5 PART 1
DOIs
StatePublished - May 1 2011

Fingerprint

Enterocytes
Candida
Epithelial Cells
Morphogenesis
Host-Pathogen Interactions
Hyphae
Gastrointestinal Diseases
Wounds and Injuries
Interleukin-8
Gastrointestinal Tract
Genotype
Cytokines

Cite this

Candida species differ in their interactions with immature human gastrointestinal epithelial cells. / Falgier, Christina; Kegley, Sara; Podgorski, Heather A; Heisel, Timothy; Storey, Kathleen; Bendel, Catherine M; Gale, Cheryl A.

In: Pediatric Research, Vol. 69, No. 5 PART 1, 01.05.2011, p. 384-389.

Research output: Contribution to journalArticle

Falgier, Christina ; Kegley, Sara ; Podgorski, Heather A ; Heisel, Timothy ; Storey, Kathleen ; Bendel, Catherine M ; Gale, Cheryl A. / Candida species differ in their interactions with immature human gastrointestinal epithelial cells. In: Pediatric Research. 2011 ; Vol. 69, No. 5 PART 1. pp. 384-389.
@article{1d54b071df314beab17709fb9899a6f1,
title = "Candida species differ in their interactions with immature human gastrointestinal epithelial cells",
abstract = "Life-threatening gastrointestinal (GI) diseases of prematurity are highly associated with systemic candidiasis. This implicates the premature GI tract as an important site for invasion by Candida. Invasive interactions of Candida spp. with immature enterocytes have heretofore not been analyzed. Using a primary immature human enterocyte line, we compared the ability of multiple isolates of different Candida spp. to penetrate, injure, and induce a cytokine response from host cells. Of all the Candida spp. analyzed, C. albicans had the greatest ability to penetrate and injure immature enterocytes and to elicit IL-8 release (p < 0.01). In addition, C. albicans was the only Candida spp. to form filamentous hyphae when in contact with immature enterocytes. Similarly, a C. albicans mutant with defective hyphal morphogenesis and invasiveness had attenuated cytotoxicity for immature enterocytes (p < 0.003). Thus, hyphal morphogenesis correlates with immature enterocyte penetration, injury, and inflammatory responses. Furthermore, variability in enterocyte injury was observed among hyphal-producing C. albicans strains, suggesting that individual organism genotypes also influence host-pathogen interactions. Overall, the finding that Candida spp. differed in their interactions with immature enterocytes implicates that individual spp. may use different pathogenesis mechanisms.",
author = "Christina Falgier and Sara Kegley and Podgorski, {Heather A} and Timothy Heisel and Kathleen Storey and Bendel, {Catherine M} and Gale, {Cheryl A}",
year = "2011",
month = "5",
day = "1",
doi = "10.1203/PDR.0b013e31821269d5",
language = "English (US)",
volume = "69",
pages = "384--389",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "5 PART 1",

}

TY - JOUR

T1 - Candida species differ in their interactions with immature human gastrointestinal epithelial cells

AU - Falgier, Christina

AU - Kegley, Sara

AU - Podgorski, Heather A

AU - Heisel, Timothy

AU - Storey, Kathleen

AU - Bendel, Catherine M

AU - Gale, Cheryl A

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Life-threatening gastrointestinal (GI) diseases of prematurity are highly associated with systemic candidiasis. This implicates the premature GI tract as an important site for invasion by Candida. Invasive interactions of Candida spp. with immature enterocytes have heretofore not been analyzed. Using a primary immature human enterocyte line, we compared the ability of multiple isolates of different Candida spp. to penetrate, injure, and induce a cytokine response from host cells. Of all the Candida spp. analyzed, C. albicans had the greatest ability to penetrate and injure immature enterocytes and to elicit IL-8 release (p < 0.01). In addition, C. albicans was the only Candida spp. to form filamentous hyphae when in contact with immature enterocytes. Similarly, a C. albicans mutant with defective hyphal morphogenesis and invasiveness had attenuated cytotoxicity for immature enterocytes (p < 0.003). Thus, hyphal morphogenesis correlates with immature enterocyte penetration, injury, and inflammatory responses. Furthermore, variability in enterocyte injury was observed among hyphal-producing C. albicans strains, suggesting that individual organism genotypes also influence host-pathogen interactions. Overall, the finding that Candida spp. differed in their interactions with immature enterocytes implicates that individual spp. may use different pathogenesis mechanisms.

AB - Life-threatening gastrointestinal (GI) diseases of prematurity are highly associated with systemic candidiasis. This implicates the premature GI tract as an important site for invasion by Candida. Invasive interactions of Candida spp. with immature enterocytes have heretofore not been analyzed. Using a primary immature human enterocyte line, we compared the ability of multiple isolates of different Candida spp. to penetrate, injure, and induce a cytokine response from host cells. Of all the Candida spp. analyzed, C. albicans had the greatest ability to penetrate and injure immature enterocytes and to elicit IL-8 release (p < 0.01). In addition, C. albicans was the only Candida spp. to form filamentous hyphae when in contact with immature enterocytes. Similarly, a C. albicans mutant with defective hyphal morphogenesis and invasiveness had attenuated cytotoxicity for immature enterocytes (p < 0.003). Thus, hyphal morphogenesis correlates with immature enterocyte penetration, injury, and inflammatory responses. Furthermore, variability in enterocyte injury was observed among hyphal-producing C. albicans strains, suggesting that individual organism genotypes also influence host-pathogen interactions. Overall, the finding that Candida spp. differed in their interactions with immature enterocytes implicates that individual spp. may use different pathogenesis mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=79954590796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954590796&partnerID=8YFLogxK

U2 - 10.1203/PDR.0b013e31821269d5

DO - 10.1203/PDR.0b013e31821269d5

M3 - Article

C2 - 21283049

AN - SCOPUS:79954590796

VL - 69

SP - 384

EP - 389

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 5 PART 1

ER -