TY - JOUR
T1 - Candida rugosa lipase alters the gastrointestinal environment in wild-type mice
AU - Menden, Ariane
AU - Hall, Davane
AU - Broedlow, Courtney Ann
AU - Darcey, Teresa
AU - Crawford, Fiona
AU - Klatt, Nichole
AU - Crynen, Stefan
AU - Mullan, Michael
AU - Ait-Ghezala, Ghania
N1 - Funding Information:
This work was supported by the Roskamp Institute and by a Sponsored Research Agreement between The Roskamp Institute and Enzymedica, Inc .
PY - 2020/10
Y1 - 2020/10
N2 - Diet and commercially available supplements can significantly impact the gut microbial composition; however, the effects of supplements often lack scientific data demonstrating the effects on healthy and diseased individuals. Hence, it was investigated, whether a frequently used supplement in humans, Candida rugosa lipase (CRL), gets delivered active beyond the stomach in the intestinal tract of C57BL/6 J mice and its impact on the gut microbial community and environment. We showed for the first time the movement of CRL in an active state through the mouse digestive tract by determination of intestinal CRL activity and free fatty acids concentrations. The short- and long-term administration of CRL resulted in significant alterations of the gut microbiome, favoring the growth of, for instance, Verrucomicrobia but also other species associated with normal body mass index (BMI) or butyrate expression, both considered beneficial. In addition, we showed that these changes persisted after supplementation and that gut barrier integrity was unaffected by the treatment. In conclusion, CRL can be delivered in an active state beyond the stomach and supplementation altered the murine gut microbiome favoring beneficial bacterial species, which may be of relevance in humans in healthy but also potentially in disease states.
AB - Diet and commercially available supplements can significantly impact the gut microbial composition; however, the effects of supplements often lack scientific data demonstrating the effects on healthy and diseased individuals. Hence, it was investigated, whether a frequently used supplement in humans, Candida rugosa lipase (CRL), gets delivered active beyond the stomach in the intestinal tract of C57BL/6 J mice and its impact on the gut microbial community and environment. We showed for the first time the movement of CRL in an active state through the mouse digestive tract by determination of intestinal CRL activity and free fatty acids concentrations. The short- and long-term administration of CRL resulted in significant alterations of the gut microbiome, favoring the growth of, for instance, Verrucomicrobia but also other species associated with normal body mass index (BMI) or butyrate expression, both considered beneficial. In addition, we showed that these changes persisted after supplementation and that gut barrier integrity was unaffected by the treatment. In conclusion, CRL can be delivered in an active state beyond the stomach and supplementation altered the murine gut microbiome favoring beneficial bacterial species, which may be of relevance in humans in healthy but also potentially in disease states.
KW - Akkermansia muciniphila
KW - Candida rugosa lipase
KW - Enzyme supplement
KW - Gut microbiome
KW - Intestinal health
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U2 - 10.1016/j.biopha.2020.110579
DO - 10.1016/j.biopha.2020.110579
M3 - Article
C2 - 32771891
AN - SCOPUS:85089072175
VL - 130
JO - Biomedicine and Pharmacotherapy
JF - Biomedicine and Pharmacotherapy
SN - 0753-3322
M1 - 110579
ER -