Candida albicans Rim13p, a protease required for Rim101p processing at acidic and alkaline pHs

Mingchun Li, Samuel J. Martin, Vincent M. Bruno, Aaron P. Mitchell, Dana A. Davis

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


Candida albicans is an important commensal of mucosal surfaces that is also an opportunistic pathogen. This organism colonizes a wide range of host sites that differ in pH; thus, it must respond appropriately to this environmental stress to survive. The ability to respond to neutral-to-alkaline pHs is governed in part by the RIM101 signal transduction pathway. Here we describe the analysis of C. albicans Rim13p, a homolog of the Rim13p/PaIB calpain-like protease member of the RIM101/pacC pathway from Saccharomyces cerevisiae and Aspergillus nidulans, respectively. RIM13, like other members of the RIM101 pathway, is required for alkaline pH-induced filamentation and growth under extreme alkaline conditions. Further, our studies suggest that the RIM101 pathway promotes pH-independent responses, including resistance to high concentrations of lithium and to the drug hygromycin B. RIM13 encodes a calpain-like protease, and we found that Rim101p undergoes a Rim13p-dependent C-terminal proteolytic processing event at neutral-to-alkaline pHs, similar to that reported for S. cerevisiae Rim101p and A. nidulans PacC. However, we present evidence that suggests that C. albicans Rim101p undergoes a novel processing event at acidic pHs that has not been reported in either 5. cerevisiae or A. nidulans. Thus, our results provide a framework to understand how the C. albicans Rim101p processing pathway promotes alkaline pH-independent processes.

Original languageEnglish (US)
Pages (from-to)741-751
Number of pages11
JournalEukaryotic Cell
Issue number3
StatePublished - Jun 2004


Dive into the research topics of 'Candida albicans Rim13p, a protease required for Rim101p processing at acidic and alkaline pHs'. Together they form a unique fingerprint.

Cite this