Cancer Stem Cell Migration in Three-Dimensional Aligned Collagen Matrices

Arja Ray, Rachel K. Morford, Paolo Provenzano

Research output: Contribution to journalArticle

Abstract

Cell migration is strongly influenced by the organization of the surrounding 3-D extracellular matrix. In particular, within fibrous solid tumors, carcinoma cell invasion may be directed by patterns of aligned collagen in the extra-epithelial space. Thus, studying the interactions of heterogeneous populations of cancer cells that include the stem/progenitor-like cancer stem cell subpopulation and aligned collagen networks is critical to our understanding of carcinoma dissemination. Here, we describe a robust method to generate aligned collagen matrices in vitro that mimic in vivo fiber organization. Subsequently, a protocol is presented for seeding aligned matrices with distinct carcinoma cell subpopulations and performing live cell imaging and quantitative analysis of cell migration. Together, the engineered constructs and the imaging techniques laid out here provide a platform to study cancer stem cell migration in 3-D anisotropic collagen with real-time visualization of cellular interactions with the fibrous matrix.

Original languageEnglish (US)
Article numbere57
JournalCurrent Protocols in Stem Cell Biology
Volume46
Issue number1
DOIs
StatePublished - Aug 1 2018

Fingerprint

Neoplastic Stem Cells
Cell Movement
Collagen
Carcinoma
Extracellular Matrix
Population
Neoplasms

Keywords

  • 3-D collagen matrices
  • cancer stem cell
  • cell migration
  • collagen alignment

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

Cite this

Cancer Stem Cell Migration in Three-Dimensional Aligned Collagen Matrices. / Ray, Arja; Morford, Rachel K.; Provenzano, Paolo.

In: Current Protocols in Stem Cell Biology, Vol. 46, No. 1, e57, 01.08.2018.

Research output: Contribution to journalArticle

@article{02936afab22345a4b7ae4b60159dc171,
title = "Cancer Stem Cell Migration in Three-Dimensional Aligned Collagen Matrices",
abstract = "Cell migration is strongly influenced by the organization of the surrounding 3-D extracellular matrix. In particular, within fibrous solid tumors, carcinoma cell invasion may be directed by patterns of aligned collagen in the extra-epithelial space. Thus, studying the interactions of heterogeneous populations of cancer cells that include the stem/progenitor-like cancer stem cell subpopulation and aligned collagen networks is critical to our understanding of carcinoma dissemination. Here, we describe a robust method to generate aligned collagen matrices in vitro that mimic in vivo fiber organization. Subsequently, a protocol is presented for seeding aligned matrices with distinct carcinoma cell subpopulations and performing live cell imaging and quantitative analysis of cell migration. Together, the engineered constructs and the imaging techniques laid out here provide a platform to study cancer stem cell migration in 3-D anisotropic collagen with real-time visualization of cellular interactions with the fibrous matrix.",
keywords = "3-D collagen matrices, cancer stem cell, cell migration, collagen alignment",
author = "Arja Ray and Morford, {Rachel K.} and Paolo Provenzano",
year = "2018",
month = "8",
day = "1",
doi = "10.1002/cpsc.57",
language = "English (US)",
volume = "46",
journal = "Current Protocols in Stem Cell Biology",
issn = "1941-7322",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Cancer Stem Cell Migration in Three-Dimensional Aligned Collagen Matrices

AU - Ray, Arja

AU - Morford, Rachel K.

AU - Provenzano, Paolo

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Cell migration is strongly influenced by the organization of the surrounding 3-D extracellular matrix. In particular, within fibrous solid tumors, carcinoma cell invasion may be directed by patterns of aligned collagen in the extra-epithelial space. Thus, studying the interactions of heterogeneous populations of cancer cells that include the stem/progenitor-like cancer stem cell subpopulation and aligned collagen networks is critical to our understanding of carcinoma dissemination. Here, we describe a robust method to generate aligned collagen matrices in vitro that mimic in vivo fiber organization. Subsequently, a protocol is presented for seeding aligned matrices with distinct carcinoma cell subpopulations and performing live cell imaging and quantitative analysis of cell migration. Together, the engineered constructs and the imaging techniques laid out here provide a platform to study cancer stem cell migration in 3-D anisotropic collagen with real-time visualization of cellular interactions with the fibrous matrix.

AB - Cell migration is strongly influenced by the organization of the surrounding 3-D extracellular matrix. In particular, within fibrous solid tumors, carcinoma cell invasion may be directed by patterns of aligned collagen in the extra-epithelial space. Thus, studying the interactions of heterogeneous populations of cancer cells that include the stem/progenitor-like cancer stem cell subpopulation and aligned collagen networks is critical to our understanding of carcinoma dissemination. Here, we describe a robust method to generate aligned collagen matrices in vitro that mimic in vivo fiber organization. Subsequently, a protocol is presented for seeding aligned matrices with distinct carcinoma cell subpopulations and performing live cell imaging and quantitative analysis of cell migration. Together, the engineered constructs and the imaging techniques laid out here provide a platform to study cancer stem cell migration in 3-D anisotropic collagen with real-time visualization of cellular interactions with the fibrous matrix.

KW - 3-D collagen matrices

KW - cancer stem cell

KW - cell migration

KW - collagen alignment

UR - http://www.scopus.com/inward/record.url?scp=85050960314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050960314&partnerID=8YFLogxK

U2 - 10.1002/cpsc.57

DO - 10.1002/cpsc.57

M3 - Article

VL - 46

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

SN - 1941-7322

IS - 1

M1 - e57

ER -