Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis

Kelsie L Becklin, Branden A. Smeester, Branden S Moriarity

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transposable elements are DNA sequences with the ability to move from one genomic location to another. The movement of class II transposable elements has been functionally harnessed and separated into two distinct DNA transposon components: the terminal inverted repeat sequences that flank genetic cargo to be mobilized and a transposase enzyme capable of recognizing the terminal inverted repeat sequences and catalyzing the transposition reaction. In particular, the Sleeping Beauty (SB) system was the first successful demonstration of transposon-based gene transfer in vertebrate species. Over the years, several improvements have been made to SB technology and more recent studies have demonstrated the versatility of the system for many applications including insertional mutagenesis, gene transfer, and transgenesis. These genetic engineering advances made available by SB both augment and advance large-scale efforts that have been directed toward identifying how genes and environmental factors influence human health in recent years. In the age of personalized medicine, the versatility of SB provides numerous genetic engineering avenues for answering novel questions in basic and applied research. This chapter discusses the use of SB-based insertional mutagenesis in mice for the efficient identification of candidate cancer genes across numerous types of cancers.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages161-170
Number of pages10
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1907
ISSN (Print)1064-3745

Fingerprint

Beauty
Neoplasm Genes
Genetic Association Studies
Mutagenesis
DNA Transposable Elements
Inverted Repeat Sequences
Genetic Engineering
Terminal Repeat Sequences
Insertional Mutagenesis
Genes
Transposases
Gene Transfer Techniques
Precision Medicine
Aptitude
Vertebrates
Technology
Health
Enzymes
Research
Neoplasms

Keywords

  • Candidate cancer gene discovery
  • Forward genetic screen
  • Insertional mutagenesis
  • Sleeping Beauty
  • Transposon

Cite this

Becklin, K. L., Smeester, B. A., & Moriarity, B. S. (2019). Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis. In Methods in Molecular Biology (pp. 161-170). (Methods in Molecular Biology; Vol. 1907). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8967-6_13

Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis. / Becklin, Kelsie L; Smeester, Branden A.; Moriarity, Branden S.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 161-170 (Methods in Molecular Biology; Vol. 1907).

Research output: Chapter in Book/Report/Conference proceedingChapter

Becklin, KL, Smeester, BA & Moriarity, BS 2019, Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1907, Humana Press Inc., pp. 161-170. https://doi.org/10.1007/978-1-4939-8967-6_13
Becklin KL, Smeester BA, Moriarity BS. Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 161-170. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8967-6_13
Becklin, Kelsie L ; Smeester, Branden A. ; Moriarity, Branden S. / Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 161-170 (Methods in Molecular Biology).
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