Cancer cell assays by use of immunocapture, subcellular imaging, and programmed cell release in gedi microdevices

Jason P. Gleghorn, Steven M. Santana, Erica D. Pratt, Matthew S. Loftus, Mona Jodari-Karimi, Neil H. Bander, David M. Nanus, Paraskevi Giannakakou, Brian J. Kirby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the use of Geometrically Enhanced Differential Immunocapture (GEDI) μdevices, which employ staggered obstacle arrays functionalized with a prostate-specific antibody, to capture and analyze prostate cancer cells on chip. GE-DI μdevices have been demonstrated to have high capture efficiency and purity [2] as compared similar devices found in the literature [4]. GEDI μdevices have been used to isolate cells from cancer patient samples and are used herein to carry out in situ chemotherapeutic efficacy experiments with a model cell line, simulating a point-of-care diagnostics situation. In addition, we also demonstrate release of captured prostate cancer cells using desthiobiotin and standard antibody conjugation chemistry.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1415-1417
Number of pages3
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Cell capture
  • Circulating tumor cells
  • Immunocapture
  • Point-of-care diagnotic

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    Gleghorn, J. P., Santana, S. M., Pratt, E. D., Loftus, M. S., Jodari-Karimi, M., Bander, N. H., Nanus, D. M., Giannakakou, P., & Kirby, B. J. (2010). Cancer cell assays by use of immunocapture, subcellular imaging, and programmed cell release in gedi microdevices. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 1415-1417). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).