CAMP-binding site of PKA as a molecular target of bisabolangelone against melanocyte-specific hyperpigmented disorder

Eunmiri Roh, Cheong Yong Yun, Ji Young Yun, Dongsun Park, Nam Doo Kim, Bang Yeon Hwang, Sang Hun Jung, Sun Ki Park, Yun Bae Kim, Sang Bae Han, Youngsoo Kim

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22 Scopus citations

Abstract

Microphthalmia-associated transcription factor (MITF) is inducible in response to cAMP and has a pivotal role in the melanocyte-specific expression of tyrosinase for skin pigmentation. Here we suggest that the cAMP-binding site of protein kinase A (PKA) is a target in the inhibition of the melanogenic process in melanocytes, as evidenced from the molecular mechanism of small molecules such as bisabolangelone (BISA) and Rp-adenosine 3′,5′-cyclic monophosphorothioate (Rp-cAMPS). BISA is a sesquiterpene constituent of Angelica koreana, a plant of the Umbelliferae family, whose roots are used as an alternative medicine. BISA competitively inhibited cAMP binding to the regulatory subunit of PKA and fitted into the cAMP-binding site on the crystal structure of PKA under the most energetically favorable simulation. In -melanocyte-stimulating hormone (-MSH)-activated melanocytes, BISA and Rp-cAMPS nullified cAMP-dependent PKA activation, dissociating catalytic subunits from an inactive holoenzyme complex. They resultantly inhibited cellular phosphorylation of the cAMP-responsive element-binding protein (CREB) or another transcription factor SOX9, thus downregulating the expression of MITF or the tyrosinase gene with decreased melanin production. Taken together, this study defined the antimelanogenic mechanism of BISA or Rp-cAMPS with a notable implication of the cAMP-binding site of PKA as a putative target ameliorating melanocyte-specific hyperpigmented disorder.

Original languageEnglish (US)
Pages (from-to)1072-1079
Number of pages8
JournalJournal of Investigative Dermatology
Volume133
Issue number4
DOIs
StatePublished - Apr 2013

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    Roh, E., Yun, C. Y., Yun, J. Y., Park, D., Doo Kim, N., Yeon Hwang, B., Jung, S. H., Park, S. K., Kim, Y. B., Han, S. B., & Kim, Y. (2013). CAMP-binding site of PKA as a molecular target of bisabolangelone against melanocyte-specific hyperpigmented disorder. Journal of Investigative Dermatology, 133(4), 1072-1079. https://doi.org/10.1038/jid.2012.425