Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry

James J. Miller; Susanta K. Hui; George S. Jackson; Sara P. Clark; Jane Einstein; Connie M. Weaver; Maryka H. Bhattacharyya

doi:10.1016/j.apradiso.2013.04.012

Calcium isolation from large-volume human urine samples for ⁴¹Ca analysis by accelerator mass spectrometry

James J. Miller
, Susanta K. Hui
, George S. Jackson
, Sara P. Clark
, Jane Einstein
, Connie M. Weaver
, Maryka H. Bhattacharyya

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Calcium oxalate precipitation is the first step in preparation of biological samples for ⁴¹Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after ⁴¹Ca administration during which human samples, collected over a lifetime, provide ⁴¹Ca:Ca ratios that are significantly above background.

Original language	English (US)
Pages (from-to)	57-61
Number of pages	5
Journal	Applied Radiation and Isotopes
Volume	78
DOIs	https://doi.org/10.1016/j.apradiso.2013.04.012
State	Published - Aug 2013

Keywords

Accelerator mass spectrometry
Biological samples
Ca analysis
Calcium oxalate
Human urine

Publisher link

10.1016/j.apradiso.2013.04.012

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Cite this

@article{139d1932c3be44aab0220eee5a98f941,

title = "Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry",

abstract = "Calcium oxalate precipitation is the first step in preparation of biological samples for 41Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after 41Ca administration during which human samples, collected over a lifetime, provide 41Ca:Ca ratios that are significantly above background.",

keywords = "Accelerator mass spectrometry, Biological samples, Ca analysis, Calcium oxalate, Human urine",

author = "Miller, \{James J.\} and Hui, \{Susanta K.\} and Jackson, \{George S.\} and Clark, \{Sara P.\} and Jane Einstein and Weaver, \{Connie M.\} and Bhattacharyya, \{Maryka H.\}",

year = "2013",

month = aug,

doi = "10.1016/j.apradiso.2013.04.012",

language = "English (US)",

volume = "78",

pages = "57--61",

journal = "Applied Radiation and Isotopes",

issn = "0969-8043",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry

AU - Miller, James J.

AU - Hui, Susanta K.

AU - Jackson, George S.

AU - Clark, Sara P.

AU - Einstein, Jane

AU - Weaver, Connie M.

AU - Bhattacharyya, Maryka H.

PY - 2013/8

Y1 - 2013/8

N2 - Calcium oxalate precipitation is the first step in preparation of biological samples for 41Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after 41Ca administration during which human samples, collected over a lifetime, provide 41Ca:Ca ratios that are significantly above background.

AB - Calcium oxalate precipitation is the first step in preparation of biological samples for 41Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after 41Ca administration during which human samples, collected over a lifetime, provide 41Ca:Ca ratios that are significantly above background.

KW - Accelerator mass spectrometry

KW - Biological samples

KW - Ca analysis

KW - Calcium oxalate

KW - Human urine

UR - https://www.scopus.com/pages/publications/84877812753

UR - https://www.scopus.com/pages/publications/84877812753#tab=citedBy

U2 - 10.1016/j.apradiso.2013.04.012

DO - 10.1016/j.apradiso.2013.04.012

M3 - Article

C2 - 23672965

AN - SCOPUS:84877812753

SN - 0969-8043

VL - 78

SP - 57

EP - 61

JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes

ER -